摘要
目的探讨胰腺癌组织和癌旁组织中FNDC5/Irisin的表达及分布情况,并分析其与临床病理特征的相关性。方法收集青岛大学附属青岛市市立医院病理科2015年1月至2018年12月手术治疗后诊断为胰腺癌的64例患者存档蜡块和2016年1月至2018年12月术中采集的30例组织样本,所收集样本均包括肿瘤组织及癌旁组织(距肿瘤边缘>2 cm)。采用实时定量PCR(qRT-PCR)和免疫组化的方法,分别检测胰腺癌组织及癌旁组织中FNDC5/Irisin的mRNA表达水平及阳性表达率,分析其与胰腺癌临床病理学特征的关系。结果应用相对定量2-△△ct法分析qRT-PCR结果表明,胰腺癌组织中FNDC5/Irisin mRNA表达水平高于相对应的癌旁胰腺组织,差异具有统计学意义(P<0.05);免疫组织化学结果表明,胰腺癌组织中FNDC5/Irisin的阳性率为59.4%,癌旁组织中FNDC5/Irisin的阳性率为28.1%,癌组织与癌旁组织中FNDC5/Irisin的阳性表达率差异有统计学意义(P<0.05);FNDC5/Irisin的表达水平与患者的性别、年龄、肿瘤大小、分化程度及肿瘤分期均无关(均P>0.05),但与肝脏的转移及淋巴结的转移相关(P<0.05)。结论胰腺癌组织中FNDC5/Irisin的高表达率明显高于癌旁组织,并与胰腺癌临床病理特征有相关性。
Objective To investigate the expression and distribution of FNDC5/Irisin in pancreatic cancer tissues and adjacent tissues,and to analyze its correlation with clinicopathological features.Methods Collection of archived wax blocks from 64 patients diagnosed with pancreatic cancer after surgical treatment from January 2015 to December 2018 in the Department of Pathology,Affiliated Qingdao Municipal Hospital of Qingdao University,and 30 tissues collected intraoperatively from January 2016 to December 2018 Samples,all collected samples included tumor tissue and corresponding adjacent tissues(>2 cm from the tumor edge).Real-time quantitative PCR(qRT-PCR)and immunohistochemistry were used to detect the expression of FNDC5/Irisin mRNA and its positivity in pancreatic cancer tissues and adjacent tissues,and to analyze its relationship with clinicopathological features of pancreatic cancer.Results qRT-PCR showed that the expression of FNDC5/Irisin mRNA in pancreatic cancer tissues was higher than that in the corresponding adjacent pancreatic tissues,the difference was statistically significant(P<0.05).The immunohistochemistry results showed that the positivity of FNDC5/Irisin in pancreatic cancer tissues was 59.4%,and the positivity of FNDC5/Irisin in adjacent tissues was 28.1%,and the positivity of FNDC5/Irisin in cancer tissues and adjacent pancreatic tissues was significantly different(P<0.05);the expression level of FNDC5/Irisin was not related with the gender,age,tumor size,degree of differentiation,and tumor stage.(P>0.05),but FNDC5/Irisin expression was associated with liver and lymph node metastasis(P<0.05).Conclusion The positivity of FNDC5/Irisin in pancreatic cancer tissues is significantly higher than that in adjacent pancreatic tissues,and it is correlated with liver and lymph node metastasis in pancreatic cancer.
作者
肖怀腾
张德国
周顺昌
赵敬熊
史光军
Xiao Huaiteng;Zhang Deguo;Zhou Shunchang;Zhao Jingxiong;Shi Guangjun(Affiliated Qingdao Municipal Hospital of Qingdao University,Qingdao 266000,China;Department of Hepatobiliary Surgery,Qingdao District People's Hospital,Qingdao 266109,China;Weifang Medical University,Weifang 261000,Shandong Province,China;Dalian Medical University,Dalian 116044,China;Department of Hepatobiliary Surgery,Affiliated Qingdao Municipal Hospital of Qingdao University,Qingdao 266071,China)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2019年第12期921-924,共4页
Chinese Journal of Hepatobiliary Surgery
基金
山东省重点研发计划项目(2018GSF118057)
青岛市科技局项目(17-3-3-40-nsh)。
