摘要
目的探讨肛周脓肿局部组织中基质细胞衍生因子1(SDF-1)和趋化因子受体4(CXCR4)的表达情况及其与患者临床病理学特征及预后的关系。方法回顾性收集2017年1月至2018年2月期间在北京市第一中西医结合医院进行手术治疗的47例肛周脓肿患者(肛周脓肿组)及58例混合痔患者(混合痔组)的临床资料。术中采集组织,采用实时定量逆转录聚合酶链式反应(qRT-PCR)法检测2组局部组织中SDF-1 mRNA和CXCR4 mRNA的表达水平,采用免疫组织化学染色法检测局部组织中SDF-1蛋白和CXCR4蛋白的表达,分析SDF-1蛋白和CXCR4蛋白的表达与肛周脓肿患者临床病理学特征以及复发的关系。结果肛周脓肿组组织中SDF-1 mRNA和CXCR4 mRNA的表达水平均相应高于混合痔组,且肛周脓肿组组织中SDF-1蛋白和CXCR4蛋白的表达阳性率也均相应高于混合痔组,差异均有统计学意义(P<0.05)。肛周脓肿患者组织中SDF-1蛋白和CXCR4蛋白的表达与患者的性别、年龄、脓肿位置及病程均无关(P>0.05),但均与脓肿直径、愈合时间及肛瘘有关(P<0.05)。SDF-1蛋白阴性组和CXCR4蛋白阴性组的术后未复发率均相应低于SDF-1蛋白阳性组及CXCR4蛋白阳性组,差异均有统计学意义(P<0.05)。结论SDF-1和CXCR4分子在肛周脓肿局部组织中的表达上调,且SDF-1蛋白和CXCR4蛋白的表达与脓肿直径、愈合时间、肛瘘形成以及复发有关。
Objective To investigate the expressions of stromal cell-derived factor-1(SDF-1)and chemokine receptor-4(CXCR4)in local tissues of perianal abscess and their relationships with clinicopathological features and prognosis of patients.Methods A total of 47 patients with perianal abscess(perianal abscess group)and 58 patients with mixed hemorrhoids(mixed hemorrhoids group)were selected for the study.The tissues were collected during the operation.Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expressions of SDF-1 mRNA and CXCR4 mRNA in local tissues of the two groups,the positive expressions of SDF-1 protein and CXCR4 protein in local tissues were detected by immunohistochemistry,and the relationships between the expressions of SDF-1 and CXCR4 protein and the clinical characteristics,prognosis of patients were analyzed.Results The expression levels of SDF-1 mRNA and CXCR4 mRNA in the perianal abscess group were higher than those in the mixed hemorrhoids group,and the positive rates of SDF-1 protein and CXCR4 protein in the perianal abscess group were higher than those in the mixed hemorrhoids group too(P<0.05).The expressions of SDF-1 protein and CXCR4 protein in perianal abscess tissues were both not related to sex,age,location of abscess,and course of disease(P>0.05),but was related to abscess diameter,healing time,and anal fistula(P<0.05).The non-recurrence rates of SDF-1 proteinnegative group and CXCR4 protein-negative group were lower than those of SDF-1 protein-positive group and CXCR4 protein-positive group respectively(P<0.05).Conclusion SDF-1 and CXCR4 molecular are up-regulated in the local tissues of perianal abscess,which are related to the size of abscess,healing time,anal fistula,and recurrence of patients.
作者
郝治
甄宏博
龚海峰
HAO Zhi;ZHEN Hongbo;GONG Haifeng(Department of General Surgery,Beijing First Hospital of Integrated Chinese and Western Medicine,Beijing 100026,P.R.China;Department of Eye,Beijing Sixth Hospital,Beijing 100007,P.R.China)
出处
《中国普外基础与临床杂志》
CAS
2019年第12期1434-1439,共6页
Chinese Journal of Bases and Clinics In General Surgery
