摘要
建立了高效液相色谱法同时测定防晒类化妆品中二乙氨基羟苯甲酰基苯甲酸己酯和甲酚曲唑三硅氧烷的方法。准确称取一定量混匀样品,经流动相(V质量分数0.1%的甲酸/水︰V甲醇︰V四氢呋喃=2︰6︰2)提取过滤后,用Welch Xtimate®C18柱(250 mm×4.6 mm×5μm)分离,再用此流动相梯度洗脱,检测波长为305 nm和355 nm。两种防晒剂在相应浓度区间线性良好(r>0.999),回收率为85%~107%,相对标准偏差<3%。该方法操作简便、结果准确、灵敏度高,适用于化妆品中两种防晒剂含量的测定。
A method for determination of diethylamino hydroxybenzoyl hexyl benzoate and drometrizole trisiloxane in sunscreen cosmetics by high performance liquid chromatography was established.A certain amount of mixing samples were weighed accurately,and dissolved with mobile phase[mass ratio of 0.1%formic acid/water-methanol-tetrahydrofuran(volume ratio 2/6/2)],then extracted and filtrated,separated on a Welch Xtimate®C18 column(4.6 mm×250 mm×5μm)at the flow rate of 1.0 mL/min by gradient elution using mobile phase.The detection wavelength were 305 nm and 355 nm.The two sunscreen agents showed a good linear correlation in certain concentration range(r>0.999),respectively.The average recoveries of 2 kinds of sunscreen agents were 85%-107%,and the relative standard deviations were less than 3%.This method is simple,accurate and highly sensitive,which is suitable for the determination of these 2 kinds of sunscreen agents.
作者
涂晓琴
杨柳
孟庆玉
殷帅
TU Xiaoqin;YANG Liu;MENG Qingyu;YIN Shuai(Hunan Institute for Drug Control,Hunan Engineering&Technology Research Center for Pharmaceutical Quality Evaluation,Changsha 410001,Hunan,China)
出处
《香料香精化妆品》
CAS
2020年第1期55-58,共4页
Flavour Fragrance Cosmetics
