摘要
目的建立化妆品中15种防晒剂的超高效液相色谱-二极管阵列检测器测定法。方法样品使用甲醇-四氢呋喃-水-高氯酸(体积比为250∶450∶300∶0.2)的混合溶液提取,采用Waters ACQUITY BEH C18色谱柱(100 mm×2.1 mm,1.7μm),0.1%甲酸甲醇-0.1%甲酸水溶液梯度洗脱,二极管阵列检测器检测。结果在2~50μg/ml的范围内,所得15种防晒剂的回归方程均呈良好的线性关系(r>0.999)。该方法的检出限为0.01~0.1μg/ml,平均回收率为91.2%~110.4%,RSD为0.6%~3.3%。结论该方法简便快捷,灵敏度高,准确度好,适用于化妆品中15种防晒剂的检测。
Objective To establish an ultra performance liquid chromatography method for determination of 15 kinds of UV filters in cosmetics.Methods The sample was extracted by the mixed liquor of methanol-tetrahydrofuran-water-perchloric acid(250∶450∶300∶0.2,V/V),then UV filters were separated by Waters ACQUITY BEH C18 column with methanol(containing0.1%formic acid)and water(containing 0.1%formic acid)as mobile phase for gradient elution,and detected by the photodiode array detector(PDA).Results In the range of 2-50μg/ml,15 kinds of UV filters showed good linearity(r>0.999).The average recovery rates were 91.2%-110.4%,the relative standard deviations were 0.6%-3.3%,the detection limits were 0.01-0.1μg/ml.Conclusion The UPLC method established in the present paper is applicable to determination of 15 kinds of UV filters in cosmetics for the advantages,such as simple operation,high sensitivity and accuracy.
作者
吕光
Lv Guang(Physical and Chemical Laboratory,Department of Health Detection,Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China)
出处
《环境与健康杂志》
CAS
北大核心
2021年第3期262-264,共3页
Journal of Environment and Health
