摘要
采用高效液相色谱-串联质谱仪(LC-MS/MS),在多反应监测(MRM)模式下,建立了同时测定化妆品中15种香豆素的快速检测方法。方法:样品经甲醇或四氢呋喃-甲醇(1:1)超声提取,过滤,CAPCELL CORE C18色谱柱(2.1 mm×100 mm,2.7μm),以乙腈(含0.2%甲酸)-0.2%甲酸为流动相,流速:0.3 mL/min;柱温:30℃;梯度洗脱,在多反应监测模式下进行定性定量分析。该方法平均回收率为83.0%~118.3%,相对标准偏差为0.9%~10.3%(n=6)方法检出限为0.05 mg/kg。在0.02~1.9 mg/L线性范围内,相关系数大于0.99。本方法专属性强,灵敏度高,适用于膏乳、水剂、粉状和精油类化妆品中的15种香豆素类化合物的检测分析。
A rapid method for the simultaneous determination of 15 coumarins in cosmetics was established by using LC-MS/MS with the multi reaction monitoring(MRM) mode.Methods:The sample was subjected to ultrasonic extraction with a mixture of tetrahydrofuran-methanol(1-1) or methanol,filtration,The chromatographic was performed using a CAPCELL CORE C18 column(2.1 mm× 100 mm,2.7 μm),with gradient elution and acetonitrile(containing 0.2 % formic acid)-0.2 % formic acid as mobile Phase,flow rate:0.3 mL/min;column temperature:30℃;qualitative and quantitative analysis in multiple reaction monitoring mode.The recoveries were 83.0 %~ 18.3 %,and the RSDs were 0.9 %~16.3 %(n=6);The method detection limits were 0.04 mg/kg.In the linear range of 0.02~1.9 mg/L,the correlation coefficient is greater than 0.99.This method has high sensitivity and specificity,and is suitable for the detection and analysis of 15 coumarins in creams,lotions,powdery sample and essential oil cosmetics.
作者
周智明
李静
Zhou Zhiming;Li Jing(Guangdong Institute for Drug Control,Guangzhou 510663,China)
出处
《广东化工》
CAS
2020年第14期159-162,共4页
Guangdong Chemical Industry
基金
中草药化妆品中合成香豆素的检测方法研究及非法添加现状调查(20182014)资助。
关键词
化妆品
高效液相色谱-质谱法
香豆素类化合物
cosmetics
high performance liquid chromatography-mass spectrometry
coumarin compounds
