摘要
目的研究不同产地赤芍的指纹图谱,测定其中没食子酸、芍药内酯苷、芍药苷、苯甲酸、苯甲酰芍药苷的含量并对其进行系统聚类,应用灰色关联度分析产地与含量的关系,为赤芍的质量评价提供参考。方法采用高效液相色谱法构建21个不同产地赤芍药材指纹图谱,并利用主成分分析及系统聚类分析对测定结果进行分类,灰色关联度法处理各指标性成分,计算其相对关联度。结果建立了21个产地赤芍的HPLC指纹图谱,标定11个共有峰,指认其中5个(没食子酸、芍药内酯苷、芍药苷、苯甲酸、苯甲酰芍药苷)共有峰,北赤芍相似度均大于0.9,川赤芍相似度均小于0.9,主成分分析结合聚类分析将其分为二类,灰色关联度分析结果显示相对关联度(ri)最大的为甘肃陇南,其次为四川甘孜。结论不同产地赤芍具有较大差异,通过建立指纹图谱,主成分结合系统聚类分析和灰色关联度分析方法对21个产地赤芍综合评价,为赤芍质量评价提供科学依据。
Objective To study the fingerprints of Paeoniae Radix Rubra in different habitats,and determine the content of five chemical components(gallic acid,albiflorin,paeoniflorin,benzoic acid,and benzoyl paeoniflorin)and systematically cluster them.The relationship between origin and content was analyzed by grey correlation degree to provide reference for the quality evaluation of Paeoniae Radix Rubra.Methods Fingerprints of Paeoniae Radix Rubra from 21 different producing areas were constructed by high performance liquid chromatography.The results were classified by principal component analysis and systematic cluster analysis.The gray correlation degree method was used to process the index components and their relative correlations were calculated.Results HPLC fingerprints of Paeoniae Radix Rubra from 21 habitats were established,11 common peaks were confirmed,and five of them(gallic acid,albiflorin,paeoniflorin,benzoic acid,and benzoyl paeoniflorin)were identified.The similarity of Paeonia lactiflora was greater than 0.9,and the similarity of Paeonia veitchii was less than 0.9.It was divided into two categories by principal component analysis combined with cluster analysis.The results of grey correlation analysis showed that the relative correlation(ri)was the largest in Gansu,followed by Ganzi in Sichuan.Conclusion There is a big difference in the relative yield of Paeoniae Radix Rubra in different habitats.This experiment provides a scientific basis for the quality evaluation of Paeoniae Radix Rubra by fingerprint analysis,principal component analysis combined system cluster analysis and grey correlation analysis method.
作者
付士朋
沈宏伟
王谦博
李俊萍
王聪
郭盛磊
王振月
FU Shi-peng;SHEN Hong-wei;WANG Qian-bo;LI Jun-ping;WANG Cong;GUO Sheng-lei;WANG Zhen-yue(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Department of Pharmacy,The First Affiliated Hospital,Guangdong Pharmaceutical University,Guangzhou 510000,China;Postdoctoral Programme of Heilongjiang Zbd Pharmaceutical Co.,Ltd.,Harbin 150040,China;Jiangyin Tianjiang Pharmaceutical Co.,Ltd.,Jiangyin 214400,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第23期5865-5871,共7页
Chinese Traditional and Herbal Drugs
基金
国家“十三五”重点研发计划（2016YFC0500303）—东北森林区道地林药资源生态开发利用技术与示范黑龙江省国家科技重大专项和重点研发项目省级资助（GX17C006）
黑龙江省博士后资助项目（LBH-Z17208）
关键词
赤芍
指纹图谱
系统聚类分析
灰色关联度分析
没食子酸
芍药内酯苷
芍药苷
苯甲酸
苯甲酰芍药苷
Paeoniae Radix Rubra
fingerprint
systematic cluster analysis
grey correlation analysis
gallic acid
albiflorin
paeoniflorin
benzoic acid
benzoyl paeoniflorin
