摘要
We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed dormancy.The seeds of P.anomala subsp.veitchii were treated with different concentrations of GA_(3)(0,100,200,300,and 400 mg/L)under dark conditions at 16℃,and then the rooting indicators including rooting rate,rooting vigor,rooting index and mold rot rate were determined.The treatment with 400 mg/L GA_(3) for 4 h showed the best performance in breaking the hypocotyl dormancy.Specifically,the treatment shortened the rooting time to 12 d,increased the rooting rate,rooting vigor,and rooting index to 84.00%±5.29%,80.67%±5.03%,and 2.25±0.16 respectively,and decreased the mold rot rate to 0.67%±1.16%.To sum up,there were germination inhibitors in the seeds of P.anomala subsp.veitchii,while the addition of exogenous hormone GA_(3) can release the inhibitory effect on the hypocotyl,which indicated that GA_(3) can promote the breaking of hypocotyl dormancy.
以川赤芍种子为材料,在16℃黑暗条件下用不同浓度赤霉素(0,100,200,300,400 mg/L)进行浸种处理,分别测定种子生根率、生根势、生根指数及霉烂率等指标,研究外源赤霉素对解除川赤芍种子下胚轴休眠的影响。结果表明:川赤芍种子用外源赤霉素浸种处理后,川赤芍种子的生根势、生根率、生根指数均显著提高;其中400 mg/L的赤霉素处理解除下胚轴休眠的效果最好,第一粒种子生根时间可缩短至12 d,生根率、生根势、生根指数分别高达为84.00%±5.29%、80.67%±5.03%、2.25±0.16,霉烂率为0.67%±1.16%。综上所述,针对川赤芍种子中可能存在的萌发抑制物,添加外源赤霉素能有效解除川赤芍种子下胚轴萌发抑制效应并对其萌发有一定促进作用。
