摘要
目的研究妊娠期Ⅱ型固有淋巴细胞相关分子与抗磷脂抗体(anti-phospholipid antibody,ACA)表达及对妊娠结局的影响。方法收集2014年1月至2017年12月河北省人民医院就诊的产妇作为研究对象,采用酶联免疫吸附法对全部入组产妇测定血清抗心磷脂抗体IgA/G/M和抗β2糖蛋白I抗体IgA/G/M,将血清抗心磷脂抗体IgA/G/M阳性产妇作为对照组,将血清抗心磷脂抗体IgA/G/M和抗β2糖蛋白I抗体IgA/G/M同时阳性作为研究组。检测产妇血清Ⅱ型固有淋巴细胞相关分子相对表达量,统计妊娠结局,分析妊娠期Ⅱ型固有淋巴细胞相关分子与抗磷脂抗体表达与不良妊娠结局的相关性。结果研究组产妇Ⅱ型固有淋巴细胞相关分子IL-33 mRNA、ST2 mRNA、GATA3 mRNA相对表达量均低于对照组,差异具有统计学意义(P<0.05)。研究组产妇总不良妊娠结局发生率显著高于对照组,差异具有统计学意义(P<0.05)。IL-33与习惯性流产正相关,与早产、死胎、胎盘早剥无关;ST2与习惯性流产、死胎呈正相关,与早产、胎盘早剥无关;GATA3与习惯性流产、早产、死胎、胎盘早剥均呈正相关;ACA-IgA、ACA-IgG、ACA-IgM与习惯性流产、早产、胎盘早剥均呈现为正相关;β2-GP1-IgA、β2-GP1-IgG、β2-GP1-IgM抗体与习惯性流产、早产、死胎、胎盘早剥均呈现为正相关。结论妊娠期Ⅱ型固有淋巴细胞相关分子与抗磷脂抗体表达与妊娠不良结局具有一定的相关性,早期检测IL-33、ST2、GATA3、ACA-IgA/G/M、β2-GP1-IgA/G/M可对妊娠不良结局进行预防和控制。
Objective To study the expression of typeⅡ intrinsic lymphocyte-associated molecules and antiphospholipid antibody(ACA)during pregnancy and its effects on pregnancy outcomes. Methods The patients who received maternal care in Hebei People′s Hospital from January 2014 to December 2017 were enrolled. Serum anticardiolipin antibody IgA/G/M and anti-β-2 glycoprotein Ⅰ antibody IgA/G/M were determined by ELISA. The parturients with postive serum anti-cardiolipin antibody IgA/G/M were taken as control group. The parturients with serum anti-cardiolipin antibody IgA/G/M and anti-β2 glycoprotein I antibody IgA/G/M both positive were taken as as study group. To detect the relative expression of serum typeⅡ intrinsic lymphocyte-related molecules in pregnant women,and to analyze the relationship between the expression of typeⅡ intrinsic lymphocyte-related molecules and anti-phospholipid antibody and adverse pregnancy outcomes. Results The relative expression levels of IL-33,ST2 and GATA3 in the study group were significantly lower than those in the control group(P < 0.05). The incidence of total adverse pregnancy outcomes in the study group was significantly higher than control group(P < 0.05). IL-33 was positively correlated with habitual abortion,but not with preterm birth,stillbirth and placental abruption. ST2 was positively correlated with habitual abortion and stillbirth,but not with preterm birth and placental abruption.GATA3 was positively correlated with habitual abortion,preterm birth,stillbirth and placental abruption. ACA IgA,ACA IgG and ACA IgM were positively correlated with habitual abortion,preterm birth and placental abruption. β2-gp1-iga and β2-gp1-igg β 2-gp1-igm antibody were positively correlated with habitual abortion,premature delivery,stillbirth and placental abruption. Conclusion The expression of typeⅡ intrinsic lymphocyte-related molecules and anti-phospholipid antibodies during pregnancy were all correlated with adverse pregnancy outcomes. Early detection of IL-33,ST2,GATA3,ACA-Ig
作者
崔佳
郭莹
高伟
王霞
王娜
帖彦清
CUI Jia;GUO Ying;GAO Wei;WANG Xia;WANG Na;TIE Yanqing(*Department of Laboratory,Hebei General Hospital,Shijiazhuang 050051,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第24期3823-3826,共4页
The Journal of Practical Medicine
基金
河北省医学科学研究课题项目(编号：20190329)
关键词
Ⅱ型固有淋巴细胞相关分子
抗磷脂抗体
妊娠期
妊娠结局
Type Ⅱ intrinsic lymphocyte-related molecules
antiphospholipid antibody
pregnancy
pregnancy outcomes
