摘要
目的探讨循环肿瘤细胞(CTCs)的检测在胃癌患者中的临床价值。方法选取2016年12月至2017年12月该院收治的178例胃癌患者为研究对象,并按照胃癌分期进行分组,其中Ⅰ期34例,Ⅱ期44例,Ⅲ期48例,Ⅳ期52例。选择同期40例健康体检者为对照组。使用CellSearch系统对各期患者、对照组及患者治疗前后外周血中CTCs计数进行检测。结果与对照组比较,胃癌患者CTCs计数升高,差异有统计学意义(P<0.05);治疗前,Ⅲ~Ⅳ期患者CTCs计数[(20.24±6.38)/7.5mL]高于Ⅰ~Ⅱ期患者[(13.56±3.28)/7.5mL],差异有统计学意义(P<0.05);与手术前比较,手术后8h的CTCs计数降低,差异有统计学意义(P<0.05)。Ⅰ~Ⅲ期患者术后8h的CTCs计数与术后24h比较,差异有统计学意义(P<0.05)。与化疗前相比,化疗1个疗程后胃癌患者CTCs计数显著降低,差异有统计学意义(P<0.05);对于更换化疗方案的患者,其CTCs计数与更换方案前比较,差异无统计学意义(P>0.05)。结论CTCs检测不仅能早期提示癌灶的转移,也为实时评估疗效及预后提供参考。
Objective To investigate the effect of detection of circulating tumor cells(CTCs)on the therapeutic effect of gastric cancer.Methods A total of 178 patients with gastric cancer were selected from December 2016 to December 2017.And there were 34 cases,44 cases,48 cases and 52 cases in stageⅠ,Ⅱ,Ⅲ and Ⅳstage.In addition,40 healthy people were selected as control group.CellSearch System was used to detect CTCs in peripheral blood of each group before and after treatments.Results The CTCs counts in gastric cancer patients were significantly higher than that of control group(P<0.05).Before treatment,the CTCs counts ofⅢ-Ⅳstage patients[(20.24±6.38)/7.5 mL]were higher than that ofⅠ-Ⅱstage patients[(13.56±3.28)/7.5 mL],and difference was statistically significant(P<0.05).The CTCs counts in patients 8 hafter surgery were lower than before surgery(P<0.05),and there were significant differences between 8 h and 24 h after surgery inⅠ-Ⅱ stage patients(P<0.05).The CTCs counts in gastric cancer patients after one course of chemotherapy were lower than before chemotherapy(P<0.05).There was no significant difference between the number of CTCs counts before and after changing chemotherapy(P>0.05).Conclusion The detection of CTCs could not only prompt the metastasis of cancer,but also provide a new way to evaluate the therapeutic effect and condition in real time.
作者
乔维洲
朱翠屏
杨婷婷
QIAO Weizhou;ZHU Cui ping;YANG Tingting(Department of Clinical Laboratory,Dalian Munici pal Central Hospital,Dalian,Liaoning 116033,China)
出处
《检验医学与临床》
CAS
2019年第24期3566-3568,共3页
Laboratory Medicine and Clinic
基金
辽宁省大连市医药卫生科学研究项目（20161109）
