摘要
目的探讨胃癌患者外周血循环肿瘤细胞(CTCs)水平及其与临床病理特征的关系。方法选取本院2013年7月至2014年1月经病理组织学证实的胃癌患者41例(胃癌组),清晨空腹抽取其肘正中静脉血5ml,24h内采用免疫磁微粒阴性富集CTCs并行免疫荧光原位杂交检测,计算CTCs阳性率并分析其与临床病理特征(TNM分期、T分期、N分期、远处转移、肿瘤大小、分化程度及脉管侵犯)的关系。选取同期的24例良性胃病患者作为对照(对照组)。结果对照组外周血CTCs阳性率为8.3%(2/24),低于胃癌组的63.4%(26/41),差异有统计学意义(P<0.05)。胃癌患者CTCs水平与TNM分期、T分期、N分期、远处转移、肿瘤大小、分化程度及脉管侵犯均无关(P>0.05)。结论胃癌患者外周血中CTCs水平较高,且其表达与胃癌临床病理特征无关,胃癌早期阶段外周血中便可检出CTCs,提示可能在肿瘤早期阶段即发生微转移。
Objective To explore the level of circulating tumor cells（ CTCs） in peripheral blood of patients with gastric cancer（ GC） and its significance with clinicopathological characteristics of GC. Methods Forty-one patients with GC from July 2013to January 2014 of our hospital were enrolled as GC group. Venous blood of 5ml was taken in the morning from the median cubital vein. The CTCs in peripheral blood were purified by negative selection using immunomagnetic beads. The CTCs were detected by immunofluorescence in situ hybridization. The positive rate of CTCs was measured and its relationships with clinicopathological characteristics（ TNM stage,T stage,N stage,pathological stage,tumor size,degree of differentiation and vascular invasion） of GC were also investigated. Meanwhile,24 patients with benign gastric diseases were chosen as control group. Results The positive rate of CTCs in peripheral blood of control group was 8. 3%（ 2 /24）,lower than 63. 4%（ 26 /41） of GC group with significant difference（ P〈0. 05）.The positive rate of CTCs in peripheral blood was no correlated with TNM stage,T stage,N stage,pathological stage,tumor size,degree of differentiation and vascular invasion. Conclusion The positive rate of CTCs in GC patients was high and there was no significant relationship between positive rate of CTCs and clinicopathologic characteristics of GC. The CTCs can be detected at the early stage of GC,indicating that micrometastases at the early stage of tumor.
出处
《临床肿瘤学杂志》
CAS
2014年第6期539-542,共4页
Chinese Clinical Oncology
关键词
胃癌
外周血
循环肿瘤细胞
临床病理特征
Gastric cancer（GC）
Peripheral blood
Circulating tumor cell
Clinicopathological characteristics
