摘要
目的探讨尿红细胞(RBC)和尿隐血(BLD)检测之间的相关关系。方法每日留取标本(88例)在科宝全自动尿液流水线上完成干化学及尿红细胞的检测,随后采用胶体金单克隆抗体法检测尿隐血,并采用人工显微镜法计数尿红细胞。依据仪器检测结果,将标本分为尿隐血阳性组(54例)和尿隐血阴性而尿沉渣仪红细胞计数异常组(34例)。同时选择其中54例干化学法检测尿液隐血阳性患者,对其标本进行煮沸后测定尿隐血。结果科宝尿液干化学隐血阳性的标本胶体金法检测结果均为阳性,54例干化学法检测尿液隐血阳性的标本煮沸后测尿隐血仅有23例阳性(显微镜镜检25例红细胞异常),假阳性率为53%。34例干化学法检测尿液隐血阴性科宝沉渣阳性患者,显微镜人工镜检阳性数为24例。结论尿中易热酶是造成尿隐血假阳性的重要原因;胶体金法灵敏度高于干化学法,阴性预测值较高,在尿隐血与尿沉渣仪红细胞结果不符时,有一定的鉴别价值。尿隐血试验假阴性的主要原因是红细胞中血红蛋白的过氧化物酶反应性降低,使干化学呈假阴性;仪器法尿红细胞计数假阳性的原因主要为草酸钙结晶和尿淋巴细胞的干扰。所以干化学测隐血和尿沉渣检查红细胞联合使用是筛查血尿的常规方法,其检测结果在疾病诊断中具有重要临床意义。
Objective To evaluate the correlation between urine red blood cells(RBC) and test of urine desiccating chemistry examination. Methods A total of 88 urine specimens were collected and analyzed using COBIO urinalysis work station. The parameters obtained from COBIO included RBC and BLD. The samples were detected by manual microscopic analysis and experimental method of monoclonal antibody. The specimens were divided into two groups according to the test results, there were 54 cases whose test of BLD was positive in one group and there were 34 cases whose test of BLD and RBC were negative and positive respectively in the other. 54 patients whose result of urine desiccating chemistry examination was negative, were detected by COBIO again after being boiled. Results All samples whose test of urine desiccating chemistry examination were positive using COBIO were positive by experimental method of monoclonal antibody. 54 samples whose test of urine desiccating chemistry examination was positive did testing occult blood by desiccating chemistry after being boiled, among them 23 samples were positive, and 25 samples were found red blood cells under the microscope. 34 samples by COBIO urinalysis work station whose test of BLD and RBC were negative and positive respectively did test under the microscope,among them 24 samples were positive. Conclusion The important reason of false negative rate using urine strips is that these is an unstable enzyme in urine. Sensitivity of experimental method of monoclonal antibody is higher than urine strips and the experimental method of monoclonal has higher negative predictive value. The monoclonal antibody immunity method has the important distinction diagnosis value, when the result could not be explained by microscopic examining and desiccating chemistry examination. The main reason why the urine occult blood is false negative is that the peroxidase in the red blood cells has decreased its reactive, which makes the chemical false negative. The main reason for the false positive of the
作者
殷贤斌
朱晓丽
黄小玲
朱翔
谢萍
方中涛
许大海
赵偲
Yin Xianbin;Zhu Xiaoli;Huang Xiaoling;Zhu Xiang;Xie Ping;Fang Zhongtao;Xu Dahai;Zhao Cai(Maanshan Center for Clinical Laboratory,Maanshan,243000,Anhui,China)
出处
《贵州医药》
CAS
2019年第11期1704-1706,共3页
Guizhou Medical Journal
基金
马鞍山市科技计划项目(2018-09)
关键词
尿隐血
尿红细胞显微镜检查
单克隆胶体金法
肾脏病
Urine occult blood
Urinary RBC detection
Monoclonl antibody immunity
Kidney disease
