摘要
目的 探讨续苓健骨汤含药血清对MC3T3-E1成骨细胞分化及增殖的影响。 方法 制备续苓健骨汤含药血清,实验分为空白对照组、含药血清低剂量组、中剂量组和高剂量组。采用CCK-8法和流式细胞术检测续苓健骨汤含药血清对MC3T3-E1细胞增殖和细胞周期的影响;碱性磷酸酶(ALP)活性测定MC3T3-E1细胞的成骨分化能力;茜素红染色检测MC3T3-E1细胞的矿化能力;实时荧光定量PCR检测成骨分化基因Runx2、OC、Bmp2、Col1a1mRNA水平。 结果 与空白对照组比较,中、高剂量续苓健骨汤含药血清能促进 MC3T3-E1 细胞增殖、S期细胞比率和细胞增殖指数,并且呈现一定的剂量依赖性;同时中高剂量续苓健骨汤含药血清组能明显提高 MC3T3-E1 细胞ALP活性( P <0.01)和钙化能力( P <0.01),促进 Runx2、OC、Bmp2、Col1a1 mRNA的表达( P <0.05)。 结论 续苓健骨汤含药血清能促进成骨细胞MC3T3-E1的增殖,并通过上调骨形成相关基因Runx2、OC、BMP2、Col1a1的表达水平,提高MC3T3-E1细胞的成骨能力。
Objective To study the effect of Xuling strong bone decoction containing serum on the proliferation and differentiation of MC3T3-E1 cells. Methods The serum containing Xuling strong bone decoction medium was prepared. The experiment was divided into blank control group, drug-containing serum low-dose group, medium-dose group, and high-dose group. CCK-8 method and flow cytometry were used to detect the effect on the proliferation and cell cycle of MC3T3-E1 cells. Alkaline phosphatase (ALP) activity was examined to determine the osteogenic differentiation ability of MC3T3-E1 cells. The mineralization of MC3T3-E1 cells was detected with Alizarin red staining. The expression levels of osteogenic differentiation genes Runx2, OC, BMP2, and Col1a1 were detected using real-time fluorescent quantitative PCR. Results Compared with those in the blank control group, the proliferation, S phase cell ratio, and cell proliferation index of MC3T3-E1 cells were promoted in the low-dose and high-dose group in a dose-dependent manner. ALP activity ( P <0.01) and calcification ability ( P <0.01) were significantly increased in high-dose group. Also, the mRNA expressions of Runx2, OC, BMP2, and Col1a1 were promoted ( P <0.05). Conclusion In a certain concentration range, Xuling strong bone decoction containing serum promotes the proliferation and differentiation of MC3T3-E1 osteoblasts.
作者
陈娟
陈赛楠
叶云金
李生强
谢丽华
黄景文
葛继荣
CHEN Juan;CHEN Sainan;YE Yunjing;LI Shengqiang;XIE Lihua;HUANG Jingwen;GE Jirong(Fujian Academy of Traditional Chinese Medicine,Fuzhou 350003,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2019年第11期1545-1549,1584,共6页
Chinese Journal of Osteoporosis
基金
福建省属科技厅公益类科研院所基本科研专项(2015R1035-11,2018R1035-15)
福建省卫生系统中青年骨干人才培养项目(2015-ZQN-JC-36)
关键词
中医中药
续苓健骨
含药血清
MC3T3-E1细胞
细胞增殖
成骨分化
traditional Chinese medicine
Xuling strong bone decoction
drug containing serum
MC3T3-E1
proliferation
differentiation
