摘要
目的探讨六味地黄丸含药血清对MC3T3-E1细胞增殖以及对Runx2、FOXO1 mRNA表达的影响。方法制作六味地黄丸混悬液,对成年Wistar大鼠进行灌胃,获取六味地黄丸含药血清。常规培养MC3T3-E1细胞24 h后更换含10%不同浓度含药血清的培养基,分别培养48 h和72 h后用CCK-8法检测MC3T3-E1细胞的增殖;对MC3T3-E1细胞进行诱导培养22天后进行饥饿培养24h,随后更换含10%不同浓度的含药血清,培养72 h后检测Runx2、FOXO1 mRNA表达。结果六味地黄丸含药血清能促进MC3T3-E1细胞增殖,并且呈现一定的剂量依赖性;同时六味地黄丸含药血清各剂量组均能明显促进Runx2 mRNA的表达(P<0.01),高剂量组的表达明显高于低剂量组(P<0.01)。高剂量组的FOX01 mRNA表达明显高于其他3组(P<0.01)。结论六味地黄丸含药血清能促进MC3T3-E1细胞增殖,同时能促进Runx2、FOXO1 mRNA表达。
Objective To investigate the effects of medicated serum of Liuwei Dihuang Pill on the proliferation of MC3T3-E1 cells and mRNA expressions of Runx2 and FOXO1. Methods The suspension of Liuwei Dihuang Pill was prepared. The adult Wistar rats were treated with the suspension of Liuwei Dihuang Pill by intragastric administration, and then the medicated serum of Liuwei Dihuang Pill was collected. After conventional culture of MC3T3-E 1 cells for 24 hours, the culture medium was replaced by 10% medicated serum with different concentrations. The proliferation of MC3T3-E1 cells was detected by CCK-8 after incubated for 48 and 72 hours respectively. The MC3T3-E1 ceils were starved for 24 hours after culture with osteoblast-inducer for 22 days, and then the culture medium was replaced by 10% medicated serum with different concentrations. After cultured for 72 hours, the mRNA expressions of Runx2 and FOXO1 were detected. Results The medicated serum of Liuwei Dihuang Pill could promote the proliferation of MC3T3- E1 cells in a certain dose- dependent manner. Meanwhile, the mRNA expression of Runx2 was significantly increased in the medicated serum of Liuwei Dihuang Pill groups with different doses （ P〈0.01 ）, and the expression in the high-dose group was significantly higher than that in the low-dose group （P〈0.01）. The mRNA expression of FOXO1 in the high-dose group was significantly higher than that in the other three groups （P〈0.01）. Conclusion The medicated serum of Liuwei Dihuang Pill can promote MC3T3-E1 cells proliferation and increase the mRNA expressions of Runx2 and FOXO1.
作者
陶乐维
陆灏
TAO Lewei;LU Hao(Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《上海中医药杂志》
2018年第9期65-68,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81302925)
上海市"杏林新星"计划项目(ZYSNXD011-RC-XLXX-20130015)
