摘要
为了探究不同肥料配比对黄芪有效成分合成途径的关键基因表达量的影响,以1年生黄芪为试材,选取3种诱导子(水杨酸、萘乙酸、硝普钠)以及固氮菌T16、T21和黄芪绿肥配制成肥料,按照正交试验设计4因素3水平的9个组合,利用实时荧光定量PCR对PAL、CHS和SS基因在不同配比施肥条件下黄芪根、茎部位的表达量进行测定分析。结果表明,不同肥料配比差异性地影响PAL、CHS、SS基因的表达量。因而,推断其可能会影响黄芪药效成分在根茎部位的合成与积累。
To explore the effect of different fertilizer ratios on the key gene expression of the active constituents of Astragalus membranaceus,one-year-old Astragalus membranaceus was used as a test material,and the three kinds of inducer(salicylic acid,naphthalene acetic acid,sodium nitrate),nitrogen-fixing bacteria T16,T21,and Astragalus membranaceus green fertilizer were formulated into fertilizers.According to orthogonal design,the 9 combinations of 4 factors and 3 levels were used to analyze the expression levels of PAL,CHS and SS genes in roots and stems of Astragalus membranaceus under different fertilization conditions by real-time fluorescent quantitative PCR.The results showed that different ratios of fertilizers could affect the expression levels of PAL,CHS and SS genes,and we concluded that it may affect the synthesis and accumulation of the active constituents of Astragalus membranaceus in roots and stems.
作者
陈家炜
梁华
李文倩
张莹
胡瑜辉
柴智
赵建平
梁建萍
CHEN Jiawei;LIANG Hua;LI Wenqian;ZHANG Ying;HU Yuhui;CHAI Zhi;ZHAO Jianping;LIANG Jianping(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China;Astragalus Resources Industrialization and Industrial Internationalization Collaborative Innovation Center,Shanxi University of Chinese Medicine,Jinzhong 030619,China)
出处
《山西农业科学》
2019年第10期1751-1755,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-004)
山西省农业科技成果转化和推广示范项目(SXNKTG201810)
山西省大学生创新创业训练计划项目(J100525517)
181校专学生科研基金项目(2018143)
关键词
实时荧光定量PCR
有效成分
基因表达差异
real-time fluorescent quantitative PCR
effective components
gene expression differences
