摘要
目的:探讨高温对甘草鲜根次生代谢的影响及其机制。方法:采用35℃、45℃、55℃温度处理甘草鲜根,研究高温胁迫对代谢途径变化、抗氧化酶活性、黄酮类成分积累的影响。结果:在高温胁迫下,各处理组甘草根中H2O2含量无明显变化;35℃和45℃组的SOD和POD活性先降低后升高,55℃组的SOD活性总体上持续降低;CAT活性未表现明显降低;1,3-二磷酸甘油酸含量显著增加。35℃条件下PAL活性较高,45℃和55℃条件下PAL活性变化不大,L-苯丙氨酸总体上维持稳定水平。不同高温条件下前3、4天均表现出芹糖基甘草苷、芹糖基异甘草苷、甘草素、异甘草素含量增加,而甘草苷、异甘草苷含量总体上呈降低趋势。结论:黄酮类成分与甘草抗氧化酶活性体系的协同作用,起到了清除活性氧保护植物的作用,前期以次生代谢产物黄酮为主,后期共同发挥作用。次生代谢产物黄酮消除活性氧主要是通过改变各成分之间的比例来调节消除活性氧的能力。
Objective:To explore the effect of high temperature on the secondary metabolism in fresh roots of Glycyrrhiza uralensis.Methods:By 35℃,45℃and 55℃temperature processing fresh licorice root,the effects of high temperature stress on metabolic pathway,antioxidant enzyme activity and flavonoid accumulation were studied.Results:Under high temperature stress,the H2O2 content in the root of Glycyrrhiza uralensis in each treatment group had no significant changes,SOD and POD activities in 35℃and 45℃group increased after lower first,SOD activity continued to decrease in 55℃group;CAT activity was not significantly decreased;1,3-diphosphate glyceric acid content increased significantly.Under the condition of 35℃,the activity of PAL was higher,under the condition of 45℃and 55℃,PAL activity had a little change,L-phenylalanine remained stable.Under different high temperature conditions,the contents of celery liquiritin,celery isoliquiritin,glycyrrhizin and isoglycyrrhizin were increased,while the contents of liquiritin and isoliquiritin were decreased.Conclusion:The synergistic effect of flavonoids and glycyrrhiza antioxidant enzyme activity system plays the role of reactive ROS to protect plants.Flavonoids,a secondary metabolite,play a major role in the early stage and play a role in the later stage.The ability of ROS elimination by secondary metabolite flavonoids is mainly regulated by changing the ratio of components.
作者
高慧如
王佳慧
关瑜
杜虹韦
孟祥才
GAO Hui-ru;WANG Jia-hui;GUAN Yu;DU Hong-wei;MENG Xiang-cai(Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中药材》
CAS
北大核心
2019年第3期524-529,共6页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81573523)
关键词
甘草
活性氧
高温
黄酮
Glycyrrhiza uralensis Fisch
Reactive oxygen species
High temperature
Flavonoids
