摘要
为明确AKR基因在葡萄非生物胁迫中的作用,利用生物信息学方法对葡萄AKR基因家族(VvAKRs)进行了全基因组鉴定,并验证其在非生物胁迫下的表达规律。结果表明:(1)该基因家族在葡萄基因组中有9个成员,主要分布在5条染色体上;氨基酸残基在275~2686 aa之间,理论等电点在5.1~9.1之间。(2)系统进化分析表明,该基因家族分为6个亚族,第6亚族VvAKR家族成员最多。(3)密码子偏好性分析结果表明,葡萄AKR基因家族密码子偏好性较弱。(4)共线性分析表明,葡萄9个AKR基因中只有VvAKR 8和VvAKR 9之间存在共线性关系。(5)qRT-PCR分析结果显示,葡萄AKR家族基因在根、茎、叶不同组织中对激素和非生物胁迫的响应程度有差异。非生物胁迫下,VvAKR 1、VvAKR 3、VvAKR 8和VvAKR 9基因在葡萄根、茎、叶组织中表达量较高;激素处理下,根组织中VvAKR 3、VvAKR 6和VvAKR 8基因在ABA、MeJA、SA处理下表达量较高;茎组织中VvAKR 3基因在NAA、GA 3处理下表达量较高;叶组织中VvAKR 1基因在各激素处理下表达量都较高。研究认为,葡萄AKR基因家族在响应葡萄非生物胁迫时发挥着不同的作用,为葡萄抗逆性研究提供了一定的理论依据。
In order to clarify the role of AKR gene in grape under abiotic stress,we identified the whole genome of grape AKR gene family(VvAKRs)by bioinformatics method,and verified its expression rule under abiotic stress.The results showed that:(1)there were 9 members of this gene family in the grape genome,mainly distributed on 5 chromosomes,the amino acid residues ranged from 275 to 2686 aa,and the theoretical isoelectric point ranged from 5.1 to 9.1.(2)Phylogenetic analysis showed that the gene family was divided into 6 subgroups,and the sixth subgroup had the most VvAKR family members.(3)The codon bias results showed that the codon bias of grape AKR gene family was weak.(4)The collinearity analysis showed that among the nine AKR genes,only the correlation between VvAKR 8 and VvAKR 9 was collinearity.(5)The results of qRT-PCR analysis showed that the responses of grape AKR family genes to hormone and abiotic stress were different in different tissues of roots,stems and leaves.Under abiotic stress,VvAKR 1,VvAKR 3,VvAKR 8 and VvAKR 9 genes were highly expressed in grape roots,stems and leaves;under hormone treatment,VvAKR 3,VvAKR 6 and VvAKR 8 genes were highly expressed in root tissues under ABA,MeJA and SA treatments;VvAKR 3 genes were highly expressed in stem tissues under NAA and GA 3 treatments;and VvAKR 1 genes were highly expressed in leaf tissues under various hormone treatments.This experimental study shows that grape AKR gene family played different roles in response to grape abiotic stress,which provided a theoretical basis for the study of grape stress resistance.
作者
周雪婷
梁国平
卢世雄
郭丽丽
陈佰鸿
毛娟
ZHOU Xueting;LIANG Guoping;LU Shixiong;GUO Lili;CHEN Baihong;MAO Juan(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第11期1851-1861,共11页
Acta Botanica Boreali-Occidentalia Sinica
基金
甘肃农业大学“伏羲杰出人才培育计划”(Gaufx-03J02)。
