摘要
目的探讨沉默信息调节蛋白4(SIRT4)在2型糖尿病肾病患者中表达水平变化的意义。方法收集30例健康体检者和90例不同尿白蛋白/肌酐比值(UACR)的糖尿病患者外周血,采用实时定量PCR和Western blotting检测SIRT4的表达水平。结果实时PCR和Western blotting结果显示,糖尿病患者体内SIRT4的表达水平较健康体检者降低(P<0.05),且随着UACR的升高而降低(r=-0.915,P<0.001)。相关分析结果显示,SIRT4与糖尿病病程(P<0.001)、体质量指数(P=0.001)、总胆固醇(P=0.009)、甘油三酯(P<0.001)、血肌酐(P<0.001)、UACR(P<0.001)、胱抑素C(P<0.001)呈负相关,与估算肾小球滤过率呈正相关(P<0.001)。结论外周血SIRT4与糖尿病肾病有一定的相关性,可能作为糖尿病肾病的早期诊断指标之一,并在反映糖尿病肾病患者肾脏功能方面也具有一定的临床参考价值。
Objective To investigate the significance of the expression level of sirtuin 4(SIRT4)in patients with type 2 diabetic kidney disease.Methods We collected peripheral blood samples from 30 healthy subjects and 90 diabetic patients with different urine albu-min-to-creatinine ratio(UACR).Real-time quantitative polymerase chain reaction(PCR)and Western blotting were used to detect SIRT4 expression level in the peripheral blood samples.Results The results of the real-time PCR and Western blotting showed that the SIRT4 expression level in diabetic patients was lower than that in healthy subjects(P<0.05)and decreased with the increase in UACR(r=-0.915,P<0.001).Correlation analysis revealed that SIRT4 expression level negatively correlated with diabetes duration(P<0.001),body mass index(P=0.001),UACR(P<0.001),and levels of total cholesterol(P=0.009),triglyceride(P<0.001),serum creatinine(P<0.001),and cystatin C(P<0.001).SIRT4 expression level positively correlated with estimated glomerular filtration rate(P<0.001).Conclusion Peripheral blood SIRT4 is correlated with and may be one of the early diagnostic indicators of diabetic kidney disease.Moreover,it has clinical reference value as an indicator of the renal function of patients with diabetic kidney disease.
作者
曹翠平
张耀杰
朱成聪
张瑜
CAO Cuiping;ZHANG Yaojie;ZHU Chengcong;ZHANG Yu(Department of Geriatrics,The First Hospital,China Medical University,Shenyang 110001,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2019年第9期778-781,共4页
Journal of China Medical University
基金
国家自然科学基金(81200597)
