摘要
目的研究早发型重度子痫前期(ESPE)和晚发型重度子痫前期(LSPE)患者胎盘组织中Staufen1(STAU1)的表达情况及其临床意义。方法选取2016年5月至2018年4月于我院剖宫产分娩的重度子痫前期(SPE)孕妇30例作为SPE组,其中ESPE15例,LSPE15例;另选择同期正常妊娠孕妇30例作为对照组(N组),其中ESPE对照(EN)组10例,LSPE对照(LN)组20例。采用实时PCR(RT-PCR)方法检测孕妇胎盘组织STAU1 mRNA的相对表达量,采用Western blotting方法及免疫组织化学方法检测胎盘组织中STAU1蛋白的表达及定位情况。结果实时PCR结果显示,SPE组胎盘组织中STAU1 mRNA表达水平显著低于N组,差异有统计学意义(0.844±0.074 vs 1.096±0.057,P<0.01);其中,ESPE组胎盘组织中STAU1 mRNA表达水平显著低于EN组,差异有统计学意义(0.683±0.061 vs 1.015±0.060,P<0.01),而LSPE组胎盘组织中STAU1 mRNA表达水平与LN组无统计学差异(1.005±0.124 vs 1.136±0.080,P>0.05)。Western blotting结果显示,STAU1蛋白表达于胎盘组织中,SPE组胎盘组织中STAU1蛋白的表达水平显著低于N组,差异有统计学意义(0.916±0.102 vs 1.573±0.135,P<0.001);其中ESPE组胎盘组织中STAU1蛋白表达水平显著低于EN组,差异有统计学意义(0.719±0.101 vs 2.025±0.155,P<0.001),而LSPE组胎盘组织STAU1蛋白表达水平与LN组无统计学差异(1.112±0.165 vs 1.337±0.165,P>0.05)。免疫组织化学结果显示,胎盘组织的细胞滋养细胞和合体滋养细胞中均有STAU1蛋白表达,且主要在合体滋养细胞的细胞质中表达。结论STAU1低表达可能与SPE中ESPE的发病密切相关。
Objective To investigate the expression of Staufen1(STAU1)in the placentas of patients with early-onset severe preeclamp-sia(ESPE)or late-onset severe preeclampsia(LSPE),and to compare its expression with those in the placentas of women in the same stage of pregnancy without preeclampsia.Methods Thirty pregnant women with severe preeclampsia(SPE)were selected as a severe preec-lampsia group(15 ESPE patients and 15 LSPE patients);and thirty pregnant women without complications were selected for the control group(N group)(10 cases in EN group,20 cases in LN group).RT-PCR was used to detect relative expression levels of STAU1 mRNA in the placentas.Western blotting and immunohistochemistry were used to detect the expression and localization of STAU1 protein in the placentas.Results RT-PCR and Western blotting results showed that expression of STAU1 mRNA and protein in the placentas of the SPE group were significantly lower than those in the N group(0.844±0.074 vs 1.096±0.057,P<0.01;0.916±0.102 vs 1.573±0.135,P<0.001).The difference was more pronounced in the ESPE group relative to the EN group(0.719±0.101 vs 2.025±0.155,P<0.001).Immunohistochemistry showed that STAU1 protein was expressed in cytotrophoblasts and syncytiotrophoblasts of the placentas and was mainly localized to the cytoplasm in the syncytiotrophoblasts.Conclusion Reduced expression of STAU1 may be closely related to the pathogenesis of ESPE in SPE.
作者
陈小斌
李媛媛
黄岭
乔宠
CHEN Xiaobin;LI Yuanyuan;HUANG Ling;QIAO Chong(Department of Obstetrics and Gynecology,Shengjing Hospital,China Medical University,Shenyang 110004,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2019年第9期769-773,共5页
Journal of China Medical University
基金
国家自然科学基金(81771610,81370735)
国家重点研发计划“生殖健康及重大出生缺陷防控研究”重点专项(2016YFC1000404)
盛京自由研究者基金(201706)
