摘要
目的探讨血清高迁移率族蛋白B1 (HMGB1)、可溶性CD40L (sCD40L)、脂肪型脂肪酸结合蛋白(FABP4)、髓过氧化物酶(MPO)在重度子痫前期患者中的表达及意义,为临床诊断和病情评估提供参考依据。方法 2016年1-12月,选择67例重度子痫前期患者,其中早发型组28例和晚发型组39例以及同期正常妊娠者60例作为对照组。采用酶联免疫吸附法检测血清HMGB1、sCD40L、FABP4和MPO的表达水平。结果重度子痫前期组患者血清HMGB1、sCD40L、FABP4和MPO水平为(76. 23±21. 59) mmol/L、(6. 37±2. 23) ng/L、(158. 61±36. 68)μg/L、(15. 69±1. 28) ng/L,而对照组为(26. 39±6. 38) mmol/L、(3. 25±1. 26) ng/L、(89. 76±11. 39)μg/L、(11. 29±0. 62) ng/L,两组比较差异有统计学意义(均P<0. 05)。早发型组血清HMGB1、sCD40L、FABP4和MPO均高于晚发型组,两组比较差异有统计学意义(均P<0. 05)。四项指标联合检测的ROC曲线下面积为0. 859,明显高于HMGB1、sCD40L、FABP4和MPO各个指标单独检测,差异有统计学意义(Z=7. 861,8. 061,8. 672,7. 541,均P<0. 001)。Pearson积差相关性显示,重度子痫前期患者中HMGB1、sCD40L、FABP4和MPO表达均呈显著相关性,差异有统计学意义(P<0. 01)。结论血清HMGB1、sCD40L、FABP4和MPO的表达与重度子痫前期发生、发展密切相关,可作为血清标志物用于重度子痫前期的诊断和病情评估。
Objective To explore the expression and significance of serum high mobility group box protein B1 (HMGB1),soluble CD40 L (sCD40L),fatty acid binding protein 4 (FABP4),and myeloperoxidase (MPO) in severe preeclampsia patients,provide a reference basis for clinical diagnosis and evaluation of the disease. Methods From January to December in 2016,67 severe preeclampsia patients were selected and divided into early-onset preeclampsia group (28 patients) and late-onset preeclampsia group (39 patients),60 normal pregnant women during the same period were selected as control group. ELISA was used to detect the expression levels of serum HMGB1,sCD40L,FABP4,and MPO. Results The levels of serum HMGB1,sCD40L,FABP4,and MPO in severe preeclampsia group were (76. 23±21. 59) mmol/L, (6. 37±2. 23) ng/L, (158. 61±36. 68) μg/L,and (15. 69±1. 28) ng/L,respectively. The levels of serum HMGB1,sCD40L,FABP4,and MPO in control group were (26. 39± 6. 38) mmol/L, (3. 25± 1. 26) ng/L, (89. 76± 11. 39) μg/L,and (11. 29±0. 62) ng/L,respectively. There were statistically significant differences in the levels of serum HMGB1,sCD40L,FABP4,and MPO between the two groups (P<0. 05). The levels of serum HMGB1,sCD40L,FABP4,and MPO in early-onset preeclampsia group were statistically significantly higher than those in late-onset preeclampsia group (P<0. 05). The area under ROC of combination detection of four parameters was 0. 859,which was statistically significantly higher than those of HMGB1,sCD40L,FABP4,and MPO,respectively (Z= 7. 861,8. 061,8. 672,7. 541,P<0. 001). Pearson product-moment correlation showed that there were significant correlations among the four parameters (P<0. 01). Conclusion The expressions of serum HMGB1,sCD40L,FABP4,and MPO are closely correlated with onset and development of severe preeclampsia,which can be used for diagnosis and disease assessment of severe preeclampsia as serum markers.
作者
刘烨
LIU Ye(Changxing County Maternal and Child Health Care Hospital,Huzhou,Zhejiang 313100,China)
出处
《中国妇幼保健》
CAS
2019年第2期280-283,共4页
Maternal and Child Health Care of China
