摘要
[目的]研究牡蛎蛋白肽的制备工艺及其功能活性评价。[方法]以新鲜无壳牡蛎为原料,采用胰酶酶解的方法制备牡蛎活性肽,经膜分离后使用HPLC测定其相对分子质量分布,使用断乳SD大鼠进行促进生长发育功能评价。[结果]得到均分子量为750Da的活性肽,该活性肽在灌胃给药剂量为2.0g/kg(剂量/体重)时,具有改善大鼠生长发育的功能。[结论]该研究对牡蛎肽的生产具有一定指导意义。
[Objective]The research aimed to study the preparation process of oyster protein peptide and its functional activity evaluation.[Method]The oyster active peptide was prepared by trypsin enzymatic hydrolysis using fresh oysters without shells as raw material.The oyster protein peptide were separated from the hydrolyzate by membrane,their molecular weight distribution was determined by HPLC,and the function of promoting growth and development was evaluated by SD weaning rats.[Result]The active peptide with average molecular weight of 750 Da was obtained,besides the protein peptide could improve the growth and development of rats at the dose of 2.0 g/kg(dose/body weight).[Conclusion]The study has certain guiding significance to the production of oyster peptide.
作者
王长伟
李八方
宋文山
刘楚怡
冯晓梅
WANG Chang-wei;LI Ba-fang;SONG Wen-shan(Marine Biomedical Research Institute of Qingdao,Qingdao,Shandong 266071)
出处
《安徽农业科学》
CAS
2019年第17期161-164,共4页
Journal of Anhui Agricultural Sciences
基金
山东省重点研究计划(2016YYSP003)
关键词
牡蛎蛋白肽
制备工艺
生长发育
功能活性
SD断乳大鼠
Oyster protein peptide
Preparation process
Growth and development
Functional activity
SD weaning rat
