摘要
目的观察气虚血瘀模型大鼠脾脏中主要的Ⅰ、Ⅱ相药物代谢酶活性的变化。方法取24只健康SD大鼠,随机分为空白对照组和模型组,每组12只。采用连续两周冰水游泳至力竭同时节食的方法制作气虚血瘀大鼠模型,空白对照组自由饮食且不强制游泳。观察两组大鼠的一般状态和体重变化,检测其全血黏度和血浆比黏度,并观察脾脏的胞浆液和线粒体中主要的6种Ⅰ相代谢酶(CYP1A2、CYP2C8、CYP2C9、CYP2C19、CYP2D6及CYP3A4)和2种Ⅱ相代谢酶[尿苷二磷酸葡醛酸转移酶1(UGT1)、雌激素硫酸转移酶(SULT1E1)]的活性改变。结果模型组大鼠毛发枯槁、精神萎靡、有便溏现象;与空白对照比较,模型组大鼠体重降低(P<0.01),全血黏度升高(P<0.01),脾脏胞浆液中CYP2C8、CYP2C19、CYP2D6活性均降低(P<0.05,P<0.01);线粒体中CYP1A2、CYP2D6、CYP3A4活性均降低,UGT1活性性升高(P<0.01)。结论气虚血瘀模型大鼠脾脏中的药物代谢酶特别是Ⅰ相代谢酶的活性发生改变,该改变可能与脾功能下降相关。
Objective To observe the changes of the main phaseⅠandⅡdrug metabolic enzyme activities in the spleen of rats with qi deficiency and blood stasis.Methods Twenty-four healthy SD rats were randomly divided into two groups:blank control group and model group,12 in each group.The model was established by ice-water swimming and diet for two weeks,while the rats in blank control group ate freely and swimming.The general state and body weight of rats were observed,and the whole blood viscosity and plasma specific viscosity was used as the index.The activities of the six major phaseⅠmetabolites(CYP1A2,CYP2C8,CYP2C9,CYP2C19,CYP2D6,and CYP3A4)and two phaseⅡmetabolites[uridine diphosphate glucuronosyl transferase 1(UGT1),estrogen sulfate transferase(SULT1E1)]in the spleen cytoplasm and mitochondria were determined by kits.Results Model group rats had dry and dull hair,lacking of energy and diarrhea.Compared with the blank control group,the model rats lost weight(P<0.01).The whole blood viscosity in the model group rats increased significantly(P<0.01);the activities of CYP2C8,CYP2C19,CYP2D6 in the spleen cytoplasm,CYP1A2,CYP2D6 and CYP3A4 in mitochondria decreased significantly(P<0.05,P<0.01)while the activity of UGT1 in mitochondria was significantly increased(P<0.01).Conclusion A significant metabolism enzyme activity change especially phaseⅠenzymes was found in the spleen of qi deficiency and blood stasis model rats,it might be related with an impaired spleen function.
作者
李苹
李洁
邓时贵
赵瑞芝
LI Ping;LI Jie;DENG Shi-gui;ZHAO Rui-zhi(The Second Clinical College of Guangzhou University of Chinese Medicine,Guangzhou,510006;School of Pharmaceutical Sciencet Guangzhou University of Chinese Medicine,Guangzhou,510006;Public Laboratory of the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,510006)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第8期993-996,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
气虚血瘀证
大鼠
血液流变学
脾脏
代谢酶活性
qi deficiency and blood stasis
rats
hemorheology
spleen
metabolic enzyme activity
