摘要
目的:从血液流变学和红细胞膜生物学变化探讨寒凝血瘀证在红细胞膜上的生物学差异。方法:20只Wistar大鼠,随机分为空白组及寒凝血瘀组,每组10只。寒凝血瘀组大鼠置于0～1℃冰水中8～10 min,待大鼠全身僵直后取出,连续造模14 d,第15天大鼠皮下注射盐酸肾上腺素0.8 mL.kg-1(0.8 mg.kg-1),共2次,间隔4 h,2次之间将大鼠置于冰水中浸泡5 min,末次造模后禁食不禁水12 h。次日以乌拉坦1 g.kg-1麻醉,固定,取血测定血液流变学指标以及红细胞膜组分相关指标。结果:与空白组比较,寒凝血瘀组大鼠体重显著降低;全血黏度显著增高(P<0.05);血浆黏度及血脂无显著变化;红细胞变形指数在600,800,1 000 s-1切变率下均显著降低(P<0.05);血沉K值显著升高(P<0.05);APTT(活化部分凝血活酶时间)降低极显著(P<0.01);FIB(纤维蛋白原)含量显著升高(P<0.05);TT(凝血酶时间)、PT(凝血酶原时间)无显著影响,但有降低趋势;Na+-K+-ATP酶活力、唾液酸含量及SOD(超氧化物歧化酶)活力均显著降低(P<0.05);MDA(丙二醛)含量及膜胆固醇含量有升高趋势但无显著差异;巯基含量有降低趋势,但无显著差异。结论:寒凝血瘀模型可能由于红细胞膜上Na+-K+-ATP酶、唾液酸及SOD的减少导致全血黏度及红细胞变形性发生改变。
Objective： To investigate biological difference in hemorheology and erythrocyte membrane in rats with blood stasis caused by cold stagnationl.Method： Twenty Wistar rats were randomly assigned to blank control group and cold stagnation and blood stasis group,10 rats in each group.These rats were put into ice water（0-1 ℃） for 8-10 minutes until the rats were titanic everyday,and the duration of modeling aws 14 days.On the day of 15,0.8 mL.kg-1 adrenalin hydrochloride was subcutaneously injected,the interval duration was 4 hours.Hemorheology indexes and activity of erythrocyte membrane were determined after 15 days.Result： Compared with blank control group,weight of rats in model group was decreased significantly.The whole blood viscosity was increased significantly（P ＆lt; 0.05）.There were no significant change in plasma viscosity and blood-fat.The red cell deformability index was decreased significantly（P ＆lt; 0.05）.Blood sedimentation K value and the content of fibrinogen（FIB） of rats in model group was increased significantly.Activated partial thromboplastin time（APTT） was decreased significantly while thrombin time（TT） and prothrombin time（PT） showed no significant change.Super oxide dismutase（SOD） and Na＋-K＋-ATP enzyme activity of erythrocyte membrane were decreasing and the content of sialic acid was decreased（P ＆lt; 0.05）.There was no significant change in the content of malondialdehyde（MDA） and plasma cholesterol.The content of hydrosulphonyl was no change.Conclusion： In cold stagnation and blood stasis model the decreased content of sialic acid,SOD and Na＋-K＋-ATP enzyme activity of erythrocyte can be found membrane which maybe one of the mechanism of increasing deformability of erythrocyte and improving the blood viscosity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期204-207,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173186)
黑龙江省博士后项目
黑龙江中医药大学优秀创新人才项目支持
关键词
寒凝血瘀
血液流变学
红细胞膜
生物学
cold stagnation and blood stasis
hemorheology
erythrocyte membrane
biology
