摘要
目的研究桃仁分离物对急性寒凝血瘀模型大鼠血液流变学和血常规水平的影响。方法采用间隔4小时两次皮下注射盐酸肾上腺素,将大鼠浸入冰水内5 min,复制急性寒凝血瘀模型大鼠动物模型,健康SD大鼠170只,雌雄各半,随机分17组,每组10只,分别为空白对照组、模型对照组,阳性对照组(丹参片),桃仁药物组分1(高、低),桃仁药物组分2(高、低),桃仁药物组分3(高、低),油酸组(高、低),亚油酸组(高、低),油酸、亚油酸组(高、低),油酸、亚油酸、棕榈酸组(高、低)。各组按剂量灌胃给药14 d,末次给药后,测定模型动物的血液流变学与血常规多项水平,进行统计分析。结果与空白对照组比较,模型组全血低、中、高切全血粘度及血浆粘度均明显增加(P<0.01);红细胞压积(Hct)、血沉(ESR)、红细胞聚集指数(EAI)、纤维蛋白原(Fib)、白细胞(WBC)、红细胞平均体积(MCV)、血小板体积分布宽度(PDW)指标明显升高(P<0.01);红细胞变形指数(EDI)、平均血小板体积(MPV)、血小板(PLT)指标明显降低(P<0.01)。与模型对照组比较,给药各剂量组均可使全血粘度及血浆粘度明显降低(P<0.05或P<0.01);Hct、ESR、EAI、Fib、WBC、PDW、MCV指标降低(P<0.05或P<0.01),EDI、MPV、PLT升高(P<0.05或P<0.01)。结论药物治疗组能通过降低急性寒凝血瘀模型大鼠全血低、中、高切全血粘度及血浆粘度水平;降低Hct、ESR、EAI、Fib、WBC、PDW、MCV的水平,提高大鼠血液中EDI、MPV、PLT的水平,可明显地改变急性寒凝血瘀模型大鼠的血液黏、浓、凝、聚状态。表明桃仁分离物具有显著的活血化瘀作用。
Objective: To study The Influence ofpeach kernel isolate on the blood rheology and blood levels of rats with Acute cold coagulated blood stasis model. Methods: The rats were immersed in ice water within 5 minutes,and injected of hydrochloride adrenaline subcutaneously twice,interval of 4 hours,to prepare the acute cold coagulated blood stasis model. healthy SD rats,half male and half female,were randomly divided into 17 groups. Each group 10 rats,as blank control group,model control group,positive control group( Salvia miltiorrhiza tablets),peach group 1( high and low),peach group 2( high and low),peach group 3( high and low),oleic acid group( high and low),linoleic acid group( high and low),oleic acid,linoleic acid group( high and low),oil,acid,linoleic acid,palmitic acid group( high and low). intragastric administration for 14 d. At the end of time after drug administration,blood rheology and blood levels were determined and statistical analyzed. Results: Compared with the blank control group,the the blood viscosityand plasma viscosity in low,medium,high shear increased significantly in model group,( P 0. 01); and hematocrit( HCT),erythrocyte sedimentation rate( ESR),erythrocyte aggregation index( EAI),fibrinogen( FIB),white blood cell( WBC),red blood cell average volume( MCV),platelet volume distribution width( PDW) increased significantly( P 0. 01); red cell deformability index( EDI),mean platelet volume( MPV),platelet( PLT) index decreased significantly( P 0. 01). With the model control group,the administration of each dose group can make whole blood viscosity and plasma vis cosity decreased significantly( P 0. 05 or P 0. 01); and HCT,ESR,EAI,FIB,WBC,PDW,MCV index decreases( P 0. 05 or P 0. 01),EDI,MPV,PLT increased( P 0. 05 or P 0. 01). Conclusion: Levels of EDI,MPV,PLT in blood were improved of drug treatment group by reducing the blood viscosity and plasma viscosity in low,medium,high shear in Acute col
出处
《陕西中医学院学报》
2016年第2期85-88,106,共5页
Journal of Shaanxi College of Traditional Chinese Medicine
基金
国家自然科学基金(30901970)
陕西省教育厅(14JK1204)
陕西省中医管理局(ZY05)
关键词
桃仁
脂肪酸
活血化瘀
peach kernel isolate
aliphatic acid
promoting blood circulation by removing blood stasis.