摘要
旨在构建具有毛囊、汗腺等皮肤附属器官的组织工程皮肤,为人类毛发移植和受损皮肤修复的临床应用提供新的思路。试验以山羊毛囊干细胞和真皮细胞为种子细胞,以羊膜、Ⅰ型胶原为支架材料,进行组织工程皮肤的体外构建,并将组织工程皮肤移植到山羊体内(试验组,同时以羊膜代替组织工程皮肤进行移植作为对照组),观察和检测其对受损皮肤的修复效果。试验结果表明,人工构建的组织工程皮肤经体外培养25d后,发现有毛囊结构形成;人工皮肤经扫描电镜观察,显示表皮细胞出现角质化;移植30、60、90d后的瘢痕面积显示,试验组创面恢复平整,瘢痕面积极显著小于对照组(P<0.01);移植270d后,活体组织切片检测发现,试验组真、表皮结构已发生重建,可见分化明显的基底层、毛囊结构,而对照组为致密的结缔组织。综上表明,本研究首次以山羊毛囊干细胞和毛囊真皮细胞为种子细胞,成功构建了具有毛囊器官的组织工程皮肤。
The human amniotic membrane,typeⅠcollagen were used as the scaffold,and the hair follicle stem cells and dermal cells from goat were used as the seed cells to construct the tissue engineered skin which would be have skin appendages such as hair follicles and sweat glands.Tissue engineered skin was constructed in vitro,and then was transplanted into goats to observe and test its repairing effect on damaged skin(as experimental group,and the transplantation of amniotic membrane instead of the tissue engineered skin was set as control).The results showed that hair follicle was formed when the tissue engineered skin was cultured for 25 days in vitro and the epidermal cells were keratinized by the scanning electron microscopy.The scar area was measured at 30,60,and 90 days after transplantation.Results showed that the scar area in the experimental group was significantly lower than the control(P<0.01).When transplanted for 270 days,histological section of the transplanted skin showed that the dermal,epidermal and the hair follicles formed in the experiment group,and the control group were just connective tissues.In conclusion,the tissue engineering skin with hair follicles was successfully constructed using goat hair follicle stem cells and dermal cells as seed cells.
作者
史明艳
高雪
杨学义
窦忠英
许尚忠
SHI Mingyan;GAO Xue;YANG Xueyi;DOU Zhongying;XU Shangzhong(Laboratory of Molecular Biology and Bovine Breeding,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Life Science,Luoyang Normal University,Luoyang 471934,China;Shaanxi Branch of National Stem Cell Engineering Center,Northwest A&F University,Yangling 712100,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第10期2097-2104,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
NSFC-河南人才培养联合基金项目(U1504323)
国家自然科学基金面上项目(31572376)
中国农业科学院基本科研增量项目(Y2016PT17),中国农业科学院创新工程项目(ASTIP-IAS03)
关键词
山羊
毛囊
干细胞
组织工程皮肤
goat
hair follicle
stem cells
tissue engineering skin
