摘要
目的 :用组织工程的方法培养人的全层复合皮肤。方法 :胎儿背部皮肤为取材对象 ,分离表皮细胞和成纤维细胞。将成纤维细胞种植入牛I型胶原凝胶中 ,培养 3d后 ,将表皮细胞接种在凝胶的表面 ,继续培养 2d ,然后将凝胶移至气液面进行复层化。 5d后取材 ,进行HE染色 ,光镜和透射电镜观察。结果 :皮肤具备表皮层和真皮层 ,其结构和正常皮肤的结构相似。表皮层中包括基底层、棘层、颗粒层和角质层 ,细胞和细胞之间以细胞间桥的形式连接 ,个别部位有角化珠的存在。表皮层和真皮层之间有完整的基底膜。部分真皮层中可以见到长短不等的上皮钉突。透射电镜结果表明 ,表皮层的各层细胞之间以桥粒连接。结论 :以胎儿皮肤为细胞来源、牛I型胶原为支架的全层组织工程皮肤 ,是一种良好的生物活性皮肤替代物 ,可用来修复全层皮肤缺损。
?Objective :To construct human skin with full thickness in vitro using tissue engineering techniques. Metheods: Epithelial cells and fibroblasts were isolated from the back skin of legally aborted human foetus. Fibroblasts were seeded into bovine type I collagen gel and cultured for 3 days. Epithelial cells were seeded on the surface of collagen gel and cultured for another 2 days, then the equivalent skin was exposed to air liquid interface to generate a protective cornified layer. 5 days later, equivalent skin was excised and observed under light microscope and transmission electron microscope. Results: Epithelium and dermis were observed in the equivalent skin,similar to those in normal human skin. Epithelium was made up of stratum basale, stratum spinosum, stratum granulosum and stratum corneum. The cells of different layers were connected with intercellular bridge. Horny pearl were also found in epithelium. Basement member was observed between epithelium and dermis. Epithelial spikes bristles of different length were existed in partial dermis. The results of TEM observation showed that desmosome existed between cells of different layer in epithelium. Conclusion: Human skin with full thickness can be tissue engineered with foetus back skin as the sources of epithelial cells and fibroblasts, and bovine I type collagen as carrier.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2002年第2期163-166,共4页
Journal of Practical Stomatology
基金
军队重点项目 (0 0Z1 0 0 )
