摘要
为研究橙盖鹅膏多糖的体外免疫调节活性、细胞毒性和抗肿瘤活性,本研究运用细胞学技术探究AC-1对T细胞、B细胞和RAW264.7三种免疫细胞的体外免疫调节活性;研究AC-1对小鼠成纤维细胞(L929)的细胞毒性以及对小鼠胃癌细胞(MFC)、小鼠肉瘤细胞(S180)的体外抗肿瘤活性。结果表明,AC-1能在体外显著刺激三种免疫细胞的增殖及RAW264.7细胞的吞噬,表明AC-1在增强机体免疫方面具有重要作用,进一步研究发现AC-1主要通过显著促进IgE和IgG的分泌来增强体液免疫。在浓度为5~20μg/mL时AC-1对L929细胞无显著的促进及抑制作用,说明AC-1对正常细胞无毒性;在浓度为10~20μg/mL时AC-1能显著抑制小鼠胃癌细胞(MFC)的生长,但对小鼠肉瘤细胞(S180)的抑制效果较弱,说明AC-1在体外能够直接抑制肿瘤细胞的生长,但对不同的肿瘤细胞具有不同的抑制效果。综上所述,橙盖鹅膏多糖(AC-1)在体外具有良好的免疫调节活性、抗肿瘤活性,但对不同的肿瘤细胞具有不同的抑制效果并且对正常细胞无毒性。
The purpose of this study is to investigate the immunomodulatory activities,cytotoxicity and anti-tumor activities of polysaccharide from Amanita caesarea (AC-1) in vitro.In this study,we used cytological techniques to explore the immunomodulatory activities of AC-1 on T cells,B cells and RAW264.7 cells in vitro,the cytotoxicity of AC-1 on mouse fibroblasts (L929) and the antineoplastic activities of AC-1 on mouse gastric cancer cells (MFC) and mouse sarcoma cells (S180 ) in vitro.The results showed that AC-1 could significantly stimulate the proliferation of three kinds of immune cells in vitro,and could enhance the phagocytosis activities of RAW264.7 cells,which indicated that AC-1 played an important role in enhancing immunities of the body.Further studies found that AC-1 mainly enhanced humoral immunity by promoting the secretion of Ig E and Ig G.When the concentration of AC-1 was 5-20 μg/mL,it had no obvious promotion or inhibition on L929 cells,suggesting that AC-1 was non-toxic to normal cells.AC-1 could significantly inhibit the growth of mouse gastric cancer cells (MFC) at the concentration of 10-20 μg/mL,but had a weak inhibitory effect on mouse sarcoma cells (S 180 ).This indicated that AC-1 could directly inhibit the growth of tumor cells in vitro,but had different inhibitory effects on different tumor cells.In conclusion,Amanita caesarea polysaccharide (AC-1) has good immunomodulatory activities,non-toxic to normal cells and good anti-tumor activities in vitro,however,it has different inhibitory effects on different cancer cells.
作者
朱淼
侯怡铃
唐贤
宋志强
丁祥
ZHU Miao;HOU Yi-ling;TANG Xian;SONG Zhi-qiang;DING Xiang(College of Life Sciences,China West Normal University;College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第9期1481-1490,共10页
Natural Product Research and Development
基金
四川省科技厅应用基础重点项目(2018JY0087)
四川省科技厅重点研发项目(2018NZ0055)
四川省教育厅重大培育项目(16CZ0018)
南充市科技局项目(16YFZJ0043)
关键词
橙盖鹅膏
多糖
免疫调节活性
细胞毒性
抗肿瘤活性
Amanita caesarea
polysaccharide
immunomodulatory activities
cytotoxicity
antitumor activities
