摘要
目的:探讨低相对分子质量姬松茸多糖(low molecular weight polysaccharide isolated from Agaricus blazei Murill,LMPAB)对树突状细胞C型植物血凝素1(Dectin-1),脾酪氨酸激酶(Sky)和胱天蛋白募集域蛋白9(CARD9)基因表达和T淋巴细胞增殖的影响。方法:体外培养ICR小鼠骨髓来源的树突状细胞;通过流式细胞术检测树突状细胞表面CD11c和CD86分子的表达;采用WST方法检测LMPAB增强树突状细胞对T淋巴细胞的增殖作用;应用实时定量PCR方法检测Dectin-1,Sky和CARD9 mRNA的表达水平。结果:树突状细胞表面CD11c表达98.6%,CD86表达96.97%;与正常组(4.84±0.21)比较,LMPAB+OVA组(6.59±0.19)可增强树突状细胞对T淋巴细胞的增殖作用(P<0.05);与正常组Dectin-1(6.13±0.27),Sky(0.36±0.11)和CARD9(1.85±0.20)比较,LMPAB+OVA组Dectin-1(8.25±0.19),Sky(1.63±0.16)和CARD9(4.40±0.19)明显升高,统计学具有显著意义(P<0.05)。结论:所培养的髓系细胞为树突状细胞;LMPAB可通过调控树突状细胞Dectin-1,Sky和CARD9 mRNA的表达增强树突状细胞的免疫效能。
Objective： To investigate the effect of low molecular weight polysaccharide isolated from Agaricus blazei Murill （LMPAB） on Dectin-1, Syk and CARD9 mRNA expression of dendritic cells. Method： Dendritic cells derived from bone marrow of ICR mice were cultured in vitro; the expression of CD1 lc and CD86 molecule on the surface of dendritic cells was detected by flow cytometry ; the effect of LMPAB on the proliferation of T lymphocytes stimulated by dendritic cells was detected by WST. Real-time quantitative PCR detect Dectin-1 mRNA, Sky mRNA and CARD9 mRNA expression levels. Result： CDllc and CD86 protein expression were expressed by 98.6% and 96.97% respectively; compared with the control group （4.84±0.21）, LMPAB ＋ OVA group （6.59 ± 0. 19） enhances the effect of dendritic cells on stimulating T lymphocytes＇ proliferation （P 〈0.05）. Compared with the mRNA expression of Dectin-1 （6. 13± 0.27）, Sky （0.36 ± 0. 11） and CARD9 （1.85±0.20） in the control group, Dectin-1 （8.25 ±0.19）, Sky （1.63 ±0.16）and CARD9 （4.40±0.19） mRNA expression in LMPAB ＋ OVA group was significantly increased with statistically significant （P 〈 0. 05）. Conclusion： The cells derived from bone marrow were dendritic ceils; immunizing potency could be enhanced by LMPAB regulating and controlling Dectin-1, Sky, CARD9 mRNA expression levels in Dendritic cells.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第10期180-183,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81173609)
