摘要
以鸡肺为原料,乙酸为提取剂,采用超声波辅助酸提法提取胶原蛋白,通过Box-Behnken试验设计分析法优化提取工艺。在单因素试验的基础上,以超声波功率、料液比和提取时间为因素,胶原蛋白得率为响应值,设计响应面试验,并比较常规酸提和超声波辅助酸提胶原蛋白的疏水性能、持油性能和乳化性能。结果显示,鸡肺胶原蛋白的超声波辅助酸提最佳条件为超声波功率365 W、料液比1∶90(质量体积比)、提取时间320 min,胶原蛋白得率占除杂后冻干鸡肺(干质量)的39.55%,其得率比常规酸提的提高了3.71倍;超声波辅助酸提的胶原蛋白疏水性、持油性和乳化活性分别比常规酸提的提高了64.0%、68.6%和32.4%。
Using chicken lung as raw material and acetic acid as extractant, collagen was extracted by ultrasonic assisted acid method, and the extraction technology was optimized by Box-Behnken experimental design analysis. On the basis of the single factor experiment, the ultrasonic power, the ratio of material to liquid and the extraction time were taken as factors. The yield of collagen was the response value, and the response surface test was designed. The hydrophobic properties, oil-holding properties and emulsifying properties of collagen by conventional extraction and ultrasonic assisted extraction were compared respectively. The results showed that the optimal extraction conditions of chicken lung collagen were ultrasonic power of 365 W, ratio of material to liquid of 1 ∶90, extraction time of 320 min, and the yield of collagen accounted for 39.55% of the lungs (dry weight) of freeze-dried chicken after decontamination. The yield of collagen by ultrasonic assisted extraction was 3.71 times higher than that by conventional extraction, and the hydrophobicity, oil-holding and emulsifying properties of collagen by ultrasonic assisted extraction were 64.0%, 68.6% and 32.4% higher than those by conventional extraction, respectively.
作者
杨恒
时海波
邹烨
王道营
徐为民
李鹏鹏
张新笑
YANG Heng;SHI Hai-bo;ZOU Ye;WANG Dao-ying;XU Wei-min;LI Peng-peng;ZHANG Xin-xiao(Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China)
出处
《江苏农业学报》
CSCD
北大核心
2019年第4期940-947,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家现代农业(肉鸡)产业技术体系建设专项(CARS-41)
江苏省农业科技自主创新基金项目
江苏省自然科学基金项目(BK20180300)
江苏省农业科学院基本科研业务专项资金项目
关键词
鸡肺胶原蛋白
超声波辅助提取
响应面
蛋白质功能特性
collagen of proteins from chicken lung
ultrasonic-assisted extraction
response surface
functional properties
