摘要
目的探讨补阳还五汤对脑缺血/再灌注大鼠神经干细胞移植后神经功能的保护作用。方法采用线栓法建立大脑中动脉闭塞模型。用药组于造模麻醉清醒后灌胃补阳还五汤(14.8 g·kg^-1·d^-1)。神经干细胞于造模24 h后移植到大鼠脑组织。Zea Longa神经功能行为学评分观察神经功能缺损情况;TTC染色检测脑梗死体积;尼氏染色检测尼氏体累积光密度值;免疫组织化学染色检测Bcl-2和Bax的表达。结果与假手术组相比,模型组出现神经功能缺损,脑梗死明显,尼氏体累积光密度值降低,Bcl-2/Bax降低(P<0.05);与模型组相比,补阳还五汤组、移植组、补阳还五汤+移植组神经功能缺损减轻,脑梗死体积缩小,尼氏体累积光密度值增高,Bcl-2/Bax增高(P<0.05);与移植组相比,补阳还五汤+移植组神经功能缺损减轻,脑梗死体积缩小,尼氏体累积光密度值增高,Bcl-2/Bax增高(P<0.05)。结论补阳还五汤可以增强神经干细胞移植对脑缺血/再灌注大鼠的神经保护作用。
Aim To explore the protective effect of nerve function of Buyang Huanwu Decoction on cerebral ischemia/reperfusion rats after the transplantation of neural stem cells(NSCs). Methods Thread bolt method was used to establish middle cerebral artery occlusion model. Drug groups were given Buyang Huanwu Decoction (14.8 g·kg ^-1 ·d ^-1 ) by gavage after the rats being sober. NSCs were transplanted to rat brain after making the model 24 hours later. Zea Longa neurobehavioral behavioral score was used to observe neural function defect, and TTC staining to detect the volume of cerebral infarction, and Nissl staining to detect Nissl body integrated optical density (IOD), and Immunohistochemical staining to detect expression of Bcl-2 and Bax. Results Compared with sham operation group, the nerve function defect appeared, and the volume of cerebral infarction increased significantly, the integral optical density of Nissl body was reduced, and the ratio of Bcl-2 to Bax was reduced in model group( P <0.05). Compared with model group, the nerve function defect was reduced, the volume of cerebral infarction was reduced, the integral optical density of Nissl body increased, and the ratio of Bcl-2 to Bax increased in BYHWD group, Transplant group and BYHWD+Transplant group ( P <0.05). Compared with transplant group, the nerve function defect was reduced, the volume of cerebral infarction was reduced, the integrated optical density of Nissl body increased, and the ratio of Bcl-2 to Bax increased in BYHWD+Transplant group( P <0.05). Conclusions Buyang Huanwu Decoction can enhance the neuroprotective effect after NSCs transplantation in cerebral ischemia/reperfusion rats.
作者
吴增
董贤慧
周晓红
靳晓飞
高维娟
WU Zeng;DONG Xian-hui;ZHOU Xiao-hong;JIN Xiao-fei;GAO Wei-juan(Hebei University of Chinese Medicine, Hebei Key Lab of Chinese Medicine Research on Cardio-cerebrovascular Diseases,Shijiazhuang 050200, China;Dept of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第9期1289-1295,共7页
Chinese Pharmacological Bulletin
基金
河北省政府资助临床医学优秀人才培养和基础课题研究项目([2017]46号-2)
河北省中医药管理局科研计划项目(No 2015007)
河北省教育厅科研计划资助项目(No ZD2016101)
河北省硕士研究生创新资助项目(No CXZZSS2017 136)
