摘要
目的:研究麻黄碱对大鼠大脑中动脉闭塞(MCAO)后运动功能康复的影响,并探讨其加速神经康复的分子机制。方法:体重为220—250g的雄性SD大鼠56只,随机分为假手术组,自然恢复组和治疗组。应用Koizumi线栓法建立单侧MCAO模型。术后1周,2周,3周,4周应用横木行走试验评定运动功能改善,免疫组织化学方法检测缺血周围区生长相关蛋白(GAP-43)、突触素(SYP)表达的变化。结果:横木行走试验评分显示治疗组康复速度明显高于自然恢复组;在1,2,3周时,免疫组化光密度定量测定显示治疗组GAP-43和SYP的表达水平高于自然恢复组。结论:麻黄碱可加速脑缺血后动物的运动功能恢复速度,其机制与促进脑内神经重塑和结构重建的分子表达有关。
Objective: To investigate the effects of ephedrine on motor recovery after middle cerebral artery occlusion(MCAO), and explore the molecular mechanism of ephedrine in accelerating rehabilitation in rats.Method:56 male SD rats weighted 220-250g were randomly divided into sham-operated group, natural recovery group and ephedrine treatment group. The unilateral MCAO model was induced by using Koizumi's method.Beam walking test was used to evaluate the improvement of motor function at weeks 1,2,3 and 4 after operation. The quantity of growth-associated protein 43(GAP-43) and synaptophysin around ischemia area were examined by immunohistochemical techniques.Result:The beam walking test score showed that the ephedrine treatment group recovered faster than the natural recovery group.Both GAP-43 and synaptophysin proteins statistical significantly increased in immunoreaction product determined by optical density measurements in ephedrine treatment group compared with natural recovery group.Conclusion:Treatment with ephedrine may facilitate recovery from behavioral dysfunction following brain injury in rats.The mechanisms of behavioral recovery with ephedrine treatment is associated with the enhanced expression of molecules involved in neuronal remodeling.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2005年第3期172-175,共4页
Chinese Journal of Rehabilitation Medicine
基金
重庆市医学科技计划项目基金资助(渝中医[2003]32号)
