摘要
目的:探讨益气活血方和补肾生髓方对局灶性脑缺血再灌注损伤大鼠恢复期缺血半暗带生长相关蛋白(GAP-43)和突触素(SYP)表达的影响。方法:以线栓法阻塞大鼠左侧大脑中动脉,复制局灶性脑缺血再灌注模型,缺血2小时再灌注1、2、3周,用免疫组织化学染色SABC法分别检测皮质缺血半暗带GAP-43和SYP的表达。结果:各时间点益气活血方、补肾生髓方组皮质缺血半暗带GAP-43(1周除外)和SYP表达阳性细胞平均吸收光密度显著升高,与模型组比较有显著性差异(P<0.05或0.01);在1周、2周GAP-43表达阳性细胞平均吸收光密度,益气活血方优于补肾生髓方组,两组间比较有显著性差异(P<0.01)。结论:益气活血方、补肾生髓方能通过促进皮质脑缺血半暗带GAP-43和SYP表达,有利于脑缺血后突触重塑和神经细胞轴突的再生,促进脑卒中后神经功能恢复。
Objective:To observe the effects of yiqi huoxue and bushen shengsui prescriptions on GAP-43 and SYP expression in convalescent period rats with cerebral ischemia-reperfusion.Methods:The focal ischemic models of middle cerebral artery occlusion were made by inserting nylon surgical thread into the left middle cerebral artery.The GAP-43 and SYP expression in convalescent period rats was determined by SABC immunohistochemistry method at middle cerebral artery occluded 2h and reperfused for 1,2,3 weeks.Results:The GAP-43 and SYP expression in convalescent period rats of the two Chinese medicine groups were elevated obviously,there was significant difference between the two groups and model group at 2 and 3 weeks,GAP-43 expression in yiqi huoxue prescription group was more obvious than that of bushen shengsui prescription group at 1 and 2 weeks.Conclusion:Yiqi huoxue and bushen shengsui prescriptions are good for the synaptic remodeling and axonal regeneration of focal cerebral ischemia-reperfusion damage by means of promoting the GAP-43 and SYP expression in convalescent period.
出处
《中国中医药科技》
CAS
2008年第6期418-419,共2页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金资助项目No.30672592
安徽省高校自然科学研究重点资助项目No.KJ2007A093
