摘要
利用高效液相色谱法(HPLC)分析不同花色铃儿花中花青素组分及其含量。样品经处理后,分析柱为C18柱、流动相为4%磷酸水溶液与乙腈、等梯度洗脱、检测波长520nm、外标法定量进行分析。结果表明,在玉色、白色、浅粉色、粉色、红色5个典型花色中,浅粉色铃儿花的花青素种类最多,为24种。随着铃儿花颜色的加深,花青素总含量逐渐增加,红色铃儿花花青素含量最高,达411.19μg/g。不同花色的铃儿花中,HPLC峰面积与峰高比值与芍药素-3-O-葡萄糖苷、飞燕草色素和锦葵色素含量成正相关趋势。
The anthocyanin constituents and contents in Enkianthus quinqeuflorus Lour with different colors were analyzed by high performance liquid chromatography(HPLC).After treating the samples ,the analytical column was C18,with 4% phosphoric acid aqueous solution and acetonitrile as the mobile phase,the isometric elution,520 nm of detection wavelength,and the external standard method were used for quantification.The results showed that in the jade,white,light pink,pink and red of E. quinqeuflorus Lour,the number of anthocyanin components of light pink was the highest,reaching 24.With the deepening of the color of E .quinqeuflorus Lour,the total anthocyanin content increased gradually,the highest content of which was red flower,reaching 411.19 μg/g.The ratio of peak area to height in HPLC was positively correlated with the contents of peonidin-3-O-glucoside,delphinidin chloride and malvidin chloride.
作者
王志广
张盟雨
易礼江
唐旭晓
罗勇志
张应中
林浩彬
王明怀
徐煲铧
王静
WANG Zhi-guang;ZHANG Meng-yu;YI Li-jiang;TANG Xu-xiao;LUO Yong-zhi;ZHANG Ying-zhong;LIN Hao-bin;WANG Ming-huai;XU Bao-hua;WANG Jing(Shatoujiao Forest Farm of Guangdong Province,Shenzhen Guangdong 518081;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization;Guangdong Academy of Forestry)
出处
《现代农业科技》
2019年第14期130-131,134,共3页
Modern Agricultural Science and Technology
基金
铃儿花种质资源收集与评价利用
云浮市特色林果产业科技创新服务中心建设(2016A040401008)
关键词
花青素
铃儿花
高效液相色谱法
anthocyanin
Enkianthus quinqeuflorus Lour
high performance liquid chromatography
