摘要
建立了粮食、蔬菜和水果中花青素的高效液相色谱检测方法。采用V(乙醇):V(水):V(盐酸)=2:1:1溶液超声提取粮食、蔬菜和水果中的花色苷,并于沸水浴中水解1 h,将多种花色苷水解为花青素。采用高效液相色谱-二极管阵列检测器对样品中的6种主要花青素(飞燕草素、矢车菊素、矮牵牛素、天竺葵素、芍药素和锦葵素)进行定量检测。6种花青素在浓度为0.5～50.0μg/mL时,色谱响应呈线性关系,r>0.999;当S/N=3时,飞燕草素、矢车菊素、天竺葵素、芍药素和锦葵素的仪器检出限均为0.01μg/mL,矮牵牛素的仪器检出限为0.03μg/mL。选取相应的空白基质进行回收率试验,在试剂空白、大米、黄豆、绿皮葡萄和甘蓝共5个空白基质里,添加0.5、1.0和2.0μg/mL 3个水平回收率试验,回收率在80.2%～110.2%,RSD在2.0%～12%。对市场上包括了粮食、蔬菜和水果9个品种的样品进行测试,给出了相应的检测结果。
A high performance liquid chromatographic（HPLC） method has been established for the simultaneous determination of six kinds of anthocyanidins including delphinidin,cyanidin,petunidin,pelargonidinm,peonidin and malvidin in cereals,vegetables and fruits.The anthocyanins in samples were extracted with mixture of ethanol： water： hydrochloric acid（2：1：1） under ultrasonic wave extraction,and then hydrolyzed into major anthocyanidins in boiling water 1 hour.Under the optimal conditions,the linearity of the method（r 0.999） was good over the concentration range from 0.5 to 50 μg/mL for six anthocyanidins.The limits of detection（LODs） of delphinidin,cyanidin,pelargonidinm,peonidin and malvidin were 0.01 μg/mL,and the LOD of petunidin was 0.03 μg/mL;The ranges of spiked recoveries at three levels of 0.5,1.0 and 2.0 μg/mL were 80.2%～110.2% with the relative standard deviations（RSDs） of 2.0%~12% in reagent blank and blank matrix of,rice,soybeans,green grapes and green cabbages.Finally,a total of 9 kinds of samples randomly collected from different markets were detected by the proposed method.The results showed different types and concentrations of anthocyanidins.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第12期43-47,共5页
Chinese Journal of Analysis Laboratory
基金
农业部农业行业标准项目(2010119)资助
关键词
花青素
高效液相色谱
粮食
蔬菜
水果
Anthocyanidin
High performance liquid chromatography
Cereal
Vegetable
Fruit
