摘要
目的研究异乌药内酯对人乳腺癌MCF-7细胞生长的抑制作用及其作用机制。方法体外培养MCF-7细胞,分为对照组及不同浓度异乌药内酯处理组。采用MTT实验检测异乌药内酯对MCF-7细胞增殖的影响;采用流式细胞术和TUNEL染色法检测异乌药内酯对MCF-7细胞周期、线粒体膜电位及细胞凋亡的影响;采用Western blotting法检测与细胞凋亡密切相关的蛋白表达情况。结果异乌药内酯以时间和剂量依赖性抑制MCF-7细胞增殖、促进细胞凋亡;能够将MCF-7细胞周期阻滞在G2/M期,诱导线粒体膜电位去极化;上调cleaved Caspase-3和促凋亡蛋白Bax表达,抑制抗凋亡蛋白Bcl-2表达,诱导MCF-7细胞的凋亡。结论异乌药内酯对MCF-7细胞的增殖抑制作用呈剂量和时间依赖性,其作用机制可能是通过线粒体途径诱导细胞凋亡。
Objective To study the effects and the mechanism of isolinderalactone on inhibiting the growth of human breast cancer MCF-7 cells. Methods Human breast cancer MCF-7 cells were cultured in vitro and treated respectively with indicated concentrations of isolinderalactone by cell culture technique. The proliferation rate was detected by MTT method;Flow cytometry and TUNEL assay were used to observe the effects of isolinderalactone on cell cycle, mitochondrial membrane potential and apoptosis in MCF-7 cells;The apoptosis related protein expression levels were determined by Western blotting. Results Isolinderalactone significantly inhibited the proliferation of MCF-7 cells by inducing cell apoptosis in a time and concentration dependent manner and induced cell cycle arrest at G2/M phases. The mitochondrial membrane potential was depolarized;And isolinderalactone up-regulated the expressions of apoptosis related proteins Bax and cleaved Caspase-3 and down-regulated the expressions of apoptosis related proteins Bcl-2. Conclusion Isolinderalactone shows obvious anti-cancer activities by inducing cell apoptosis. The mechanism of inducing apoptosis may be associated with the reduction of mitochondrial membrane potential and activation of caspase pathway.
作者
刘自尧
杨芳
赵崇妍
杨鹏硕
连增林
史新元
LIU Zi-yao;YANG Fang;ZHAO Chong-yan;YANG Peng-shuo;LIAN Zeng-lin;SHI Xin-yuan(School of Life Science,Beijing University of Chinese Medicine,Beijing 102488,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Yichuang Biotechnology Industry Research Institute,Beijing 101111,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第12期2922-2927,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(81473364)
北京市科委科技新星交叉学科项目(xxhz201210)
关键词
异乌药内酯
MCF-7细胞
增殖抑制
凋亡
线粒体膜电位
isolinderalactone
MCF-7 cells
proliferation inhibition
apoptosis
mitochondrial membrane potential
