摘要
目的探讨人类内源性病毒W家族囊膜蛋白合胞素(syncytin)对白血病细胞凋亡的影响及其信号机制。方法对小鼠淋巴瘤细胞EL4细胞稳转表达合胞素,显微镜观察细胞形态变化,流式细胞术检测细胞凋亡、细胞周期及线粒体膜电位情况,用试剂盒检测PKC和Caspase-3活性。结果合胞素稳定过表达后,EL4细胞形态和PKC活性无明显改变,细胞凋亡明显增加,线粒体膜电位去极化增加,Caspase-3活性增强。结论合胞素可诱导小鼠淋巴瘤细胞EL4细胞线粒体途径细胞凋亡,在白血病的发生发展过程中发挥重要作用。
Objective To explore the effect of syncytin,the envelop protein of the human endogenous virus W family,on the apoptosis of leukemia cells and its signaling mechanisms.Methods The mouse lymphoma cell line EL4 was transfected to stablely express syncytin,then the changes of cell morphology were observed with microscope;cell apoptosis,cell cycle and mitochondrial membrane potential were detected by flow cytometry;PKC and Caspase activities were detected by kit.Results After stable overexpression of syncytin,while no significant changes in the cell morphology of EL4 and the activity of PKC,there was a significant increase in the cell apoptosis,the depolarization of mitochondrial membrane potential and the activity of Caspase-3.Conclusion Syncytin may induce cell apoptosis via mitochondrial signal pathway in mouse lymphoma cell line EL4 and play an important role in the genesis and progression of leukemia.
作者
孙鹥
范欣
徐亚
井敏敏
Sun Yi;Fan Xin;Xu Ya;Jing Minmin(Department of Clinical Laboratories,The First People’s Hospital of Yunnan Province,Kunming 650032,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2018年第5期405-415,共11页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金(81100377)
云南省应用基础研究(昆医联合专项2013FB203)
云南省医学学科带头人培养项目(D-201670)
