摘要
目的采用HPLC法同时测定乌药中乌药内酯、乌药醚内酯、异乌药内酯含量的方法。方法色谱柱为KromasilC18柱(250mm×4.6mm,5μm),流动相:甲醇-乙腈-水(35:25:40);检测波长:235nm;柱温:40℃;流速:1ml/min。结果乌药内酯、乌药醚内酯、异乌药内酯的进样量分别在0.06～0.56μg(r=0.9992,n=6)、0.05～0.53μg(r=0.9992,n=6)、0.05～0.51μg(r=0.9993,n=6)的范围内与峰面积呈良好的线性关系。乌药内酯的平均回收率为99.12%,RSD为2.49%;乌药醚内酯的平均回收率为103.34%,RSD为1.59%;异乌药内酯的平均回收率为99.27%,RSD为3.58%。结论该法简单、可靠,可作为控制乌药药材质量的参考标准。
Objective To establish a method for determination of linderalactone,linderane,isolinderalactone in Radix linderae by RP-HPLC. Methods The sample was separated on Kromasil C18 （250 mm× 4.6 mm,5 μm） column, the mobile phase was ethanol-acetonitrile-water （35： 25： 40） at a flow rate of 1.0 ml/ min and the detection wavelength was 235 nm and the column temperature was 40℃. Results The calibration carves were linear in the range of 0.06 - 0. 56 μg for linderalactone （ r = 0. 999 2, n = 6 ） , 0.05 - 0. 53 μg for linderae （ r = 0. 999 2, n = 6 ） and 0. 05 - 0. 51 μg for isolinderalactone （ r = 0. 999 3, n = 6 ）. The average recoveries of linderalactone were 99. 12% ,RSD were 2.49% ;The average recoveries of linderane were 103.34% , RSD were 1.59% ;The average recoveries of isolinderalactone were 99. 27%, RSD were 3.58%. Conclusion The method is simple, accurate, reproducible and can offer referenees for the quality control of Radix linderae.
出处
《中国现代药物应用》
2009年第8期7-8,共2页
Chinese Journal of Modern Drug Application
