摘要
目的：建立用HPLC法测定乌药中异乌药内酯含量的方法。方法：色谱柱为Kromasil C18柱（250mm×4．6mm，5um），流动相：甲醇-乙腈-水（35：25：40，v／v）；检测波长：235nm；柱温：40℃；流速：1mL／min。结果：异乌药内酯的进样量在0．15μg～1．52μg（r=-0．9997，n=6）的范围内与峰面积呈良好的线性关系；平均回收率为98．81％，RSD为1．52％。结论：该法简单、可靠，可作为控制乌药药材质量的参考标准。
Objective： To establish a method for determination of isolinderalactone in Radix linderae by RP-HPLC. Method： The sample was separated on Kromasil C18 （250 mm×4.6 mm,5μm） column, the mobile phase was ethanol-acetonitrile-water （35：25：40v/v） at a flow rate of 1.0 mLomin-1 and the detection wavelength was 235 nm and the column temperature was 40℃. Results： The calibration carves were linear in the range of 0.15μg～1.52μg （r=0.9997,n=6）. The average recoveries of isolinderalactone were98.81%, RSD were 1.52%. Conclusion： The method is simple, accurate, reproducible and can offer referenees for the quality control of Radix linderae.
出处
《中医临床研究》
2010年第9期112-113,115,共3页
Clinical Journal Of Chinese Medicine
