摘要
PHD(Plant Homeodomain Finger)基因家族编码一类锌指转录因子,广泛参与植物的生长发育和逆境应答过程。通过全基因组鉴定获得了95个大豆PHD家族蛋白。通过共线性分析、进化树构建、基因结构和功能结构域鉴定、GO注释分析、不同组织间和非生物胁迫下表达分析等,获得了大豆PHD家族基因复制、家族进化、保守结构域及基因表达等信息。结果表明,大豆PHD基因在家族进化、基因结构和保守结构域上存在较大变异,可能参与Zn2+结合、DNA结合及表观遗传调控等分子过程,参与调控植物生长发育和逆境应答。这些结果为进一步研究大豆PHD家族在生长发育和逆境应答中的生物学功能提供重要线索。
PHD(Plant Homeodomain Finger) family genes encode a class of zinc finger transcription factor proteins,which are widely involved in regulating plant growth and stress responses.In this study,through genome-wide analysis,we identified a total of 95 PHD family proteins in soybean proteome.We further performed the synteny analysis,constructed the phylogenic tree,identified the gene structures and functional domains,analyzed the gene ontology(GO) annotation,and investigated the expression patterns in different tissues and under abiotic stresses.These data provided comprehensive information regarding the gene duplication,family evolution,conserved domains and gene expression of the soybean PHD family.Our results presented in this study suggest that soybean PHD family displays a great divergence in family evolution,gene structure and conserved domain organization;possibly participates in different molecular processes,including Zn2+ binding,DNA binding and epigenetic regulation;and probably controls plant development and environmental stress responses.In summary,these findings will provide a crucial cue for further characterizing the biological function of soybean PHD family in plant growth and environmental stress responses.
作者
杨珺凯
沈阳
才晓溪
邬升杨
李建伟
孙明哲
贾博为
孙晓丽
Yang Junkai;Shen Yang;Cai Xiaoxi;Wu Shengyang;Li Jianwei;Sun Mingzhe;Jia Bowei;Sun Xiaoli(College of Agronomy, Heilongjiang Bayi Agricultural University/Crop Stress Molecular Biology Laboratory, Daqing 163319, Heilongjiang, China)
出处
《作物杂志》
CAS
北大核心
2019年第3期55-65,共11页
Crops
基金
国家自然科学基金面上项目(31671596)
中国博士后科学基金特别资助项目(2017T100253)
黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y08)
关键词
大豆
PHD转录因子
PHD家族分析
表达特征
逆境胁迫
Soybean
PHD transcription factor
Gene family analysis
Expression patterns
Environmental stress
