摘要
为研究高粱HIR基因家族成员的表达特性,采用生物信息学分析方法和实时荧光定量(qRT-PCR)技术,对高粱HIR基因家族进行鉴定和分析。结果表明:高粱中有5个HIR成员(SbHIR1~SbHIR5),分布于高粱第2、3、7、9、10号染色体上;系统进化分析发现高粱与玉米的亲缘关系最近;不同物种间及物种内HIR基因的有效密码子数相对保守;基因结构分析表明,高粱HIR基因内含子数为1~6个;Motif分析表明,高粱SbHIR2蛋白缺失Motif 5和Motif 8,其余高粱HIR成员均具有9个Motif;高粱HIR蛋白二级结构以α-螺旋为主;qRT-PCR分析显示,高粱HIR基因家族成员在根、茎和叶中表达量存在差异,且能响应脱落酸、赤霉素和flg22的胁迫。
In order to study the expression characteristics of the members of the sorghum HIR gene family,this study used bioinformatics analysis methods and quantitative real-time PCR(qRT-PCR)technology to identify and analyze the sorghum HIR gene family.The results indicated that,in the sorghum genome,a total of 5 HIR members were identified,named SbHIR1~SbHIR5,which were distributed on chromosomes 2,3,7,9,and 10.Phylogenetic analysis demonstrated that sorghum and maize was closely related.The number of effective codons of HIR genes was relatively conserved among and within different species.Gene structure analysis indicated that the number of introns in sorghum HIR gene family members ranged from 1 to 6.Motif analysis indicated that sorghum SbHIR2 protein lacked Motif 5 and Motif 8,and all other sorghum HIR members had 9 Motifs.The secondary structure of sorghum HIR protein was mainly composed withα-helix.qRT-PCR analysis indicated that the expression levels of sorghum HIR gene family members were different in roots,stems and leaves,and responding to the stress of abscisic acid,gibberellin and flg22.
作者
陈俊
方远鹏
杜巧丽
蒋君梅
任明见
李向阳
田山君
谢鑫
Chen Jun;Fang Yuanpeng;Du Qiaoli;Jiang Junmei;Ren Mingjian;Li Xiangyang;Tian Shanjun;Xie Xin(Key Laboratory of Agricultural Microbiology Characteristics,College of Agriculture,Guizhou University,Guiyang 550025;Guizhou branch of National Wheat Improvement Center,Guiyang 550025;Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang 550025)
出处
《中国粮油学报》
CAS
CSCD
北大核心
2021年第7期161-168,共8页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金(32060614)
贵州省科技计划(黔科合支撑[2019]2408号/黔科合成果[2019]4246)
贵州省特色杂粮现代农业产业技术体系(GZCYTX 2018-04-3)。
