摘要
目的探讨淫羊藿苷及其代谢产物宝藿苷I对气管切开模型大鼠肺组织病理及炎症的影响。方法将72只SPF级雄性SD大鼠随机分成四组,正常对照组(A)、模型非用药组(B)、模型+宝藿苷I组(C)、模型+淫羊藿苷组(D),每组18只。制备大鼠气管切开插管留置模型。模型成功6h后给予C组宝藿苷I,D组淫羊藿苷干预,A、B组给与等量0.9%生理盐水。分别在24、72、168h取肺组织及肺泡灌洗液。肺组织用苏木精-伊红染色(HE染色),光镜下观察分析肺组织的病理学改变;ELISA检测肺泡灌洗液白细胞介素6(IL-6),肿瘤坏死因子α(TNF-α)表达。结果1)肺组织病理结果显示:气管切开后B组大鼠肺损伤程度随时间延长逐渐加重,明显高于A组(P<0.05)。C、D组肺损伤程度均明显轻于B组,且D组在24、168h均明显轻于C组(P<0.05)。D组在168h病理评分明显降低(与24h比较P<0.01)。(2)肺泡灌洗液IL-6、TNF-α含量:气管切开插管后大鼠肺泡灌洗液IL-6和TNF-α含量在三个时段均明显高于A组(P<0.05)。用宝藿苷I和淫羊藿苷干预后,C、D组IL-6、TNF-α含量在72、168h明显低于B组(P<0.05);且C、D组组内IL-6和TNF-α含量在72、168h明显低于24h(P<0.05);D组与C组相比,IL-6含量无明显统计学差异(P>0.05)。D组TNF-α在72h、168h均明显低于C(P<0.05)。结论宝藿苷I和淫羊藿苷对气管切开术后早期肺部炎症有一定疗效,其机制与抑制肺泡灌洗液炎症因子IL-6、TNF-α的表达,减轻肺组织损伤有关,且淫羊藿苷效果更佳。
Objective To investigate the effects of icariin and its metabolite,baohuoside I,on the pathology and inflammation of lung tissue in tracheotomized rats.Methods Seventy-two SPF male Sprague-Dawley rats were randomly divided into four groups: normal control group (A),model control group (B),and model treatment group (C and D) with 18 rats in each group.A rat tracheotomy tube indwelling model was established.At 6 hours after successful modeling,the groups C and D were administered icariin and baohuoside I,respectively.The groups A and B were administered an equal amount of 0.9% normal saline.Lung tissue and alveolar lavage fluid were collected at 24,72,and 168 h.Pathological examination of the lung tissues was performed using HE staining.ELISA was used to detect the interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in alveolar lavage fluid.Results After the tracheotomy,the degree of histological changes in the lung tissues of the group B was gradually increased over time,significantly higher than that in the group A ( P < 0.05).The degree of lung injury in the groups C and D was significantly lower than that in the group B,and that in the group D was significantly lower compared with the group C at 24 and 168 h after tracheotomy ( P < 0.05).The pathological score of group D was significantly lower at 168 h ( P < 0.01 compared with 24 h).The contents of IL-6 and TNF-α in alveolar lavage fluid of the rats after tracheotomy were significantly higher than those in the group A ( P < 0.05).After treatments with baohuoside I and icariin,the levels of IL-6 and TNF-α in the groups C and D were significantly lower than those in the group B at 72 and 168 h ( P< 0.05),and the IL-6 and TNF-α contents in the groups C and D at 72 h and 168 h were significantly lower than that after 24 h ( P< 0.05).There was no significant difference in the IL-6 contents of groups C and D at 72 h and 168 h ( P > 0.05).TNF-α contents in the group D at 72 and 168 h were significantly lower than that in the group C ( P < 0.05).Conclusi
作者
鞠静
彭玲玲
潘宇政
谭人千
潘茂华
韦进新
JU Jing;PENG Lingling;PAN Yuzheng;TAN Renqian;PAN Maohua;WEI Jinxin(the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Guangxi Shanglin CountyPeople's Hospital,Nanning 530500;The Fourth People's Hospital of Nanning,Nanning 530021)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2019年第3期305-310,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(81460687)~~
