摘要
目的研究输血相关性急性肺损伤（transfusion-relatedacutelunginjury，TRALI）SD大鼠肺组织细胞间黏附分子（intercellularcelladhesionmolecule，ICAM）一1基因表达和中性粒细胞（polymorphonuclearneutrophil，PMN）数量的变化，旨在探讨ICAM-1和PMN在TRALI发病中的作用。方法60只SD大鼠按随机数字表法分为正常对照组、脂多糖组、TRALI组、阳性对照组各15只。①正常对照组采用假手术处理；②脂多糖组经大鼠腹腔注射脂多糖（2mg／kg，≤1min完毕），2h后静脉输注生理盐水（约1mL／只）；（3）TRALI组腹腔注射脂多糖（2mg／kg）2h后静脉输注血浆（约1mL／只）；④阳性对照组静脉输注脂多糖（5mg／kg）诱导急性肺损伤。采用Western印迹法检测血浆CAM-1蛋白表达；采用逆转录聚合酶链反应（RT—PCR）检测大鼠肺组织中ICAM-1基因表达量；采用免疫组化法观察肺组织中ICAM一1蛋白表达。结果阳性对照组、TRALI组血浆ICAM-1蛋白表达均显著高于正常对照组、脂多糖组（0．58±0．09、0．41±0．07VS．0．12±0．03、0．21±0．04，均P〈0．01）；与正常对照组肺组织中ICAM-1蛋白含量和ICAM-1mRNA表达[（0．16±0．03）和（0．36±0．05）]比较，阳性对照组[（0．33±0．09）和（1．53±0．17）]和TRALI组[（0．27±0．07）和（1．24±0．11）]明显升高（P均〈0．05）；TRALI组BALF中细胞总数和PMN百分比[（310±76．6）×10^6／L和（33．5±11．5）％]明显高于正常对照组[（101±63．8）×10^6／L和（9．8±3．5）％，P均〈0．05）]；TRALI组组织含水量和MPO活性明显高于正常对照组（P均〈0．05）。结论TRALI大鼠肺组织中ICAM-1蛋白、ICAM-1mRNA的表达升高，肺组织PMN浸润增加，提示ICAM-1参与了PMN与内皮细胞的黏附和聚集，表明ICAM-1和PMN可能在TRALI的炎症反应过程中发挥重要作用。
Objective To study the expression of intercellular cell adhesion molecule - 1 （ ICAM - 1 ） and the number of polymorphonuclear neutrophil （PMN） in rats with transfusion - related acute lung injury,to explore their possible role in TRALI. Methods Sixty Sprague Dawley （SD） rats were randomLy divided into normal control group （ n = 15 ） with sham operation, positive control group （n = 15 ） with ALI induced by intravenous infusion of lipopolysaccharide （LPS） （5 mg/kg）, and TRALI group （n = 15 ） treated by intraperitoneal injection of LPS 2 h before the transfusion of human plasma（ about 1 mL）, LPS control group （ n = 15 ） treated by intraperitoneal injection of LPS 2 h. The reverse transcription - polymerase chain （RT - PCR） was used to detect ICAM - 1 mRNA. Andimmunohistochemical method was used to detect ICAM - I protein expression, Morphological changes in lung was observed under light microscope. Myeloperoxidase （MPO） in lung homogenate ,wet lung weight to dry lung weight ratio（W/D） were observed. Results Compared with normal control group and LPS control group, Expression of ICAM - 1 protein in plasma were increased significantly in positive control group and TRALI group（0.58 ± 0.09,0.41 ± 0.07 vs. 0.12 ± 0.03,0.21 ± 0.04 ） （ all P 〈 0.01 ） ; Compared with normal control group （0.16 ± 0.03 ） and （0.36 ±0.05 ）, positive control group（0. 33 ± 0.09 and 1.53 ± 0. 17 ） and TRALI group （0.27 ±0. 07 and 1.24 ± 0.11 ）, Expression of ICAM - 1 protein and ICAM - 1 mRNA were increased significantly in the lung （ all P 〈 0.05 ） ; TRALI group [ （ 310 ±76.6 ） × 10^6/L and （ 33.5 ± 11.5 ） % ], the number of cells in BALF and PMN percentage were significantly higher than that of normal control group[ （ 101 ± 63.8 ） × 10^6/L and （9.8 ± 3.5 ） % ] （ all P 〈 0.05 ） ;TRALI group, tissue water content and MPO activity is significantly higher than normal control group （ all P 〈 0.05 ）. Conclusion Expression o
出处
《中国急救医学》
CAS
CSCD
北大核心
2016年第1期86-90,F0003,共6页
Chinese Journal of Critical Care Medicine
关键词
急性肺损伤
输血相关性
细胞间黏附分子-1
中性粒细胞
SD大鼠
基因
Acute lung injury
transfusion - related
Intercellular cell adhesion molecules
Polymorphonuclear neutrophil
Sprague Dawley rats
Gene
