摘要
【目的】为探究不同纳米氧化铁(α-Fe2O3、γ-Fe2O3、Fe3O4)和离子铁(Fe^2+、Fe^3+)对小麦幼苗生长的影响。【方法】以小麦品种新麦18号为试验材料,使用不同纳米氧化铁和离子铁对小麦幼苗进行水培处理后,对小麦幼苗的抗氧化体系、叶绿素、根系活力等进行检测。【结果】与对照相比,纳米氧化铁和离子铁处理下的小麦的Fe含量均显著升高,小麦的叶绿素和花青素未发生显著变化。纳米Fe3O4处理下叶中SOD酶、可溶性蛋白和可溶性糖均升高;Fe^2+处理下的小麦的根和叶中MDA、叶中SOD酶、叶中POD酶、根中CAT酶活性、叶中可溶性蛋白和根中可溶性糖均显著升高;Fe^3+处理下的小麦的根长显著减少,根系活力、根和叶中MDA、根中CAT酶活性、叶中POD酶活性、根和叶中可溶性糖均显著升高。【结论】同浓度处理下,纳米材料展现了比离子更小的生物毒性和更高的生物有效性。为纳米氧化铁作为铁肥在农业上使用这一思路提供了一定的理论依据和数据基础。
【Objective】The research aimed at studying the physiological and biochemical influence of different iron oxide nanoparticles (α-Fe 2O 3,γ-Fe 2O 3, Fe 3O 4 NPs) and ions (Fe^2+, Fe^3+) on the growth of wheat seedlings.【Method】With new wheat 18 wheat varieties as experimental material, anti-oxidizing system, chlorophyll content and root activity of wheat seedlings were measured after being treated with different iron oxide NPs under hydroponic culture.【Result】After being treated with different different iron oxide NPs and equivalent ions, iron content were increased apparently compared with the control, and the content of chlorophyll and anthocyanin remained relatively stable. After being treated with Fe 3O 4 NPs , the content of soluble sugar and soluble protein were increased remarkably in leaves. After being treated with Fe^2+, the content of MDA in roots and leaves, soluble protein in leaf and soluble sugar in roots, the activity of POD in leaves and CAT in root, were significantly increased. Root length of wheat under Fe^3+ treatment was significantly decreased, however, root activity, the content of MDA and soluble sugar in roots and leaves, the activity of CAT in root and POD in leaves, were all significantly increased.【Conclusion】All the results showed that under the same concentration treatment, NPs not only showed less phytotoxicity than equivalent ions, but also had higher bioavailability, which provided certain theoretical basis and data basis for the use of iron oxide nanoparticles as iron fertilizer in agriculture.
作者
李俊丽
邹正康
刘倩
陆芹洪
杨道勇
LI Jun-li;ZOU Zheng-kang;LIU Qian;LU Qin-hong;YANG Dao-yong(Department of Biological Science and Technology, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Hubei Wuhan 430070, China)
出处
《西南农业学报》
CSCD
北大核心
2019年第5期1004-1010,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31301735)
中央高校基本科研业务费专项资金资助(2017IB006)
国家级大学生创新训练项目(20181049715007)
关键词
纳米氧化铁
小麦
生理影响
Iron oxide nanoparticles
Wheat
Physiological effects
