摘要
为分析当前猫泛白细胞减少症病毒(FPLV)基因组遗传变异情况,对FPLV胶体金试纸条检测结果为阳性的猫粪进行病毒分离,通过血凝、分子生物学等试验对分离株进行鉴定。结果表明:病毒在CRFK细胞上盲传5代后产生明显细胞病变,电镜观察病毒粒子直径约25 nm,分离病毒的血凝效价为1∶256,将分离株命名为FPLV BJ04。基因组扩增结果显示,FPLV BJ04基因组大小为5 118 bp。决定病毒宿主范围和抗原性的VP2蛋白系统进化树分析表明,FPLV BJ04株与2017年意大利分离株KX434462株位于同一分支;分离株的VP2、NS1基因与GenBank收录的FPLV VP2、NS1基因相似性分别为99.1%～99.5%和99.0%～99.5%。动物回归试验中攻毒组猫出现临床症状和病理变化。本研究对分离的FPLV北京流行株的VP2和NS1基因进行遗传变异分析,可为更好地预防和控制FPLV提供基础。
To analyze genetic variation of feline panleukopenia virus (FPLV),in this study,a fecal sample was collected from a cat infected FPLV in Beijing and was used to isolate FPLV by CRFK cells,and identified by hemagglutination assay (HA),molecular biology.The results showed that cytopathic effects could be observed in CRFK cells.The diameter of virus particle about 25 nm was observed by electron microscope.The HA blood coagulation rate was 1:256.The full genome was cloned,sequenced and analyzed.The results showed that the isolated virus had a genome of 5 118 bp,phylogenetic tree of VP2 showed that FPLV BJ04 strain and KX434462 strain were on the same small branch.The isolated virus,according to VP2 gene homology analysis,shared from 99.1% to 99.5% homology with reference strains,and NS1 gene shared from 99.0% to 99.5%.Animal regression test results also suggested that clinical symptoms and pathological changes were observed in the challenge group.In this study,an epidemic strain of FPLV in Beijing was successfully isolated,and the genetic variation of VP2 and NS1 gene were analyzed.The results provide basic information for the better prevention and control of FPLV.
作者
刘琪
史利军
梁琳
张玲玲
袁维峰
梁瑞英
李金祥
崔尚金
LIU Qi;SHI Lijun;LIANG Lin;ZHANG Lingling;YUAN Weifeng;LIANG Ruiying;LI Jinxiang;CUI Shangjin(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology of Beijing,Ministry of Agriculture,Beijing 100193,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第5期1106-1112,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十三五"国家重点研发计划(2016YFD0501003)
中国农业科学院创新工程项目(ASTIP-IAS15)
关键词
猫泛白细胞减少症病毒
病毒分离鉴定
序列分析
feline panleukopenia virus (FPLV)
isolation and identification
sequence analysis
