摘要
根据GenBank登录的猫细小病毒(CU-4)VP1基因序列,设计了1对引物,用PCR技术对VP1基因进行整体扩增,将扩增产物纯化后克隆入PGM-T载体,通过酶切、PCR和测序进行验证。结果表明,测序拼接得出VP1基因的序列长度约为2306 bp,该基因的ORF总长为2184 bp,编码727个氨基酸,应用DNAStar软件把所测得的序列与国内外代表性毒株的VP1基因编码区全长核苷酸序列和氨基酸序列进行比对分析,发现CC-1株与国内XJ-1株的核苷酸和氨基酸同源性均为99.3%。
To analyze the sequence of VP1 gene of Feline parvovirus CC-1 strain and compare with that of representative virus strain.According to the reported complete nucleotide sequence of FPV（CU-4） in GenBank,a pair of primers were designed and synthesized.VP1 gene of isolate was amplified.The products of PCR were cloned into PGM-T vector.the positive clone was identified bu enzyme cutting,PCR and sequencing.The whole sequence of VP1gene was analyzed using DNAStar software.The results indicated that the VP1 gene with 2306 bp long had a single opening read frame 2184 bp in length and coded a polypeptide of 727 amino acids. The sequence analysis demonstrated that represent reference strains loaded down from GenBank,the result of ORF sequence analysis indicated that CC-1 strain exhibited 99.3% homology of the nucleotides and amino acid with that of the XJ-1 strain in our county. To analyze the sequence of VP1 full gene,have a significance in clinic and epidemiological study.
出处
《中国畜牧兽医》
CAS
2008年第8期40-43,共4页
China Animal Husbandry & Veterinary Medicine
