摘要
2018年3月17日,贵州省某梅花鹿场的梅花鹿(Cervus nippon)出现关节肿大、下颌肿大、睾丸单侧肿胀,还伴有严重的跛行等症状。采集4份该发病梅花鹿的血样送检。检测结果表明,通过虎红平板凝集试验2份血样检测出了布氏杆菌感染,4份血样经RT-PCR均扩增出了JEV NS1部分基因的特异性条带,PCR产物测序结果分析显示,4份梅花鹿血样的JEV NS1基因(GZ-015、GZ-019、GZ-022、GZ-039)间的核苷酸同源性分别为99. 9%、99. 3%、99. 4%,与强毒株SA-14、国内外的疫苗株及四川、吉林等地的JEV分离株之间核苷酸同源性为99%以上,GZ-015、GZ-019、GZ-022、GZ-039与强毒株SA-14、弱毒株、国内外的疫苗株及四川、吉林等地的JEV分离株处于同一个较大的遗传进化分支。
On 17 March 2018,sika deer(Cervus nippon)in a sika deer farm in Guizhou Province had developed symptoms,including swollen joints,swollen jaws,unilateral swelling of the testicles,accompanied by severe lameness.The farm staff collected 4 blood samples from the affected sika deer for laboratory analyses.The test results showed that Brucella infection was detected in 2 samples by tiger red plate agglutination test,and 4 blood samples were amplified at JEV NS1 specific bands by RT-PCR.The sequencing analysis of PCR products showed that the nucleotide homologies among the JEV NS1 genes(GZ-015,GZ-019,GZ-022,and GZ-039)of the blood samples from four sika deer were 99.9%,99.3%,and 99.4%,respectively,and the virulent strain SA-14,domestic and foreign vaccine strains and the nucleotide homology between JEV isolates in Sichuan and Jilin were greater than 99%.GZ-015,GZ-019,GZ-022,GZ-039 and virulent strains SA-14,attenuated strains,vaccine strains at home and abroad,and JEV isolates from Sichuan and Jilin are in the same branch of genetic evolution.
作者
郭倩妤
石远菊
汤德元
叶丽
杨伟
廖少山
刘巧玲
GUO Qianyu;SHI Yuanju;TANG Deyuan;YE Li;YANG Wei;LIAO Shaoshan;LIU Qiaoling(College of Animal Science,Guizhou University,Guiyang,Guizhou,550025,China)
出处
《野生动物学报》
北大核心
2019年第2期435-439,共5页
CHINESE JOURNAL OF WILDLIFE
基金
贵州省2017年农业攻关项目(黔科合NY字[2017] 3051号)
贵州省教育厅创新群体重大研究项目(黔教合KY字[2017] 027)
