摘要
对谷胱甘肽体内体外的抗过敏活性进行研究。采用透明质酸酶抑制率实验、致敏豚鼠离体回肠肌过敏性收缩实验研究谷胱甘肽体外抗过敏作用;通过大鼠被动皮肤过敏反应(PCA)实验和大鼠迟发型超敏反应(DTH)实验测定谷胱甘肽的体内抗过敏活性。谷胱甘肽的浓度达5mg·mL^(-1)时,对透明质酸酶的抑制率可达到70.83%,对致敏豚鼠离体回肠肌过敏性收缩的抑制率达92.36%,且具有剂量依赖性;与致敏组大鼠相比,高剂量的谷胱甘肽组大鼠被动皮肤过敏反应的蓝斑面积显著减小,迟发型超敏反应的鼠足垫体积显著减小,与扑尔敏的抗过敏效用相似。谷胱甘肽具有较强的抗过敏活性。
The anti-allergic activities of glutathione in vitro and in vivo were investigated. The anti-allergic of glutathione in vitro was studied by using the inhibition rate of hyaluronidase assay and the test of sensitized guinea pig isolated ileum muscle. The anti-allergic efficacy of glutathione in vivo was analyzed by passive cutaneous anaphylaxis in rats (PCA) and delayed type hypersensitivity in rats (DTH). The inhibition rate of hyaluronidase reached as high as 70.83%, and the inhibition rate of allergic contraction of sensitized guinea pig isolated ileum muscle was 92.36% when glutathione concentration was 5 mg·mL -1 ,which was a concentration-dependent manner. Compared with sensitized rats, the high dose of glutathione reduced the blue-spot area of passive cutaneous anaphylaxis, as well as delayed type hypersensitivity of rat footpad volume, with the similar effect to chlorpheniramine. The glutathione possesses good anti-allergic activity.
作者
赵新玲
牛真真
公衍玲
金宏
ZHAO Xinling;NIU Zhenzhen;GONG Yanling;JIN Hong(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
出处
《青岛科技大学学报（自然科学版）》
CAS
2019年第3期23-28,共6页
Journal of Qingdao University of Science and Technology:Natural Science Edition
基金
山东省高校科技计划项目(J15LK12)
关键词
谷胱甘肽
抗过敏
透明质酸酶
过敏反应
抑制作用
glutathione
anti-allergy
hyaluronic acid enzyme
allergic reaction
inhibition
