摘要
目的探究REGγ基因异常表达与甲状腺乳头状癌(papillary thyroid carcinoma,PTC)发生、发展相关性。方法收集2015年1月—2018年6月成都市第二人民医院手术切除甲状腺标本103例,采用实时定量PCR法检测REGγmRNA,采用免疫组织化学染色检测REGγ蛋白的表达,并与临床病理资料对比分析。结果 PTC组织中REGγmRNA表达水平为(3. 58±1. 60),REGγ蛋白阳性率为66. 10%,均高于甲状腺滤泡性腺瘤、结节性甲状腺肿及癌旁正常甲状腺组织的表达,差异比较有统计学意义(P<0. 05);肿瘤直径<2 cm患者REGγmRNA表达水平及REGγ蛋白阳性率显著高于肿瘤直径≥2 cm患者,无淋巴结转移患者REGγmRNA表达水平及REGγ蛋白阳性率显著高于淋巴结转移患者,差异比较有统计学意义(P<0. 05); PTC组织中REGγmRNA检测与REGγ蛋白表达呈正相关关系(r=0. 485,P<0. 05)。结论 REGγ基因表达与PTC的发生、发展相关,可为肿瘤恶性程度、转移情况及预后评估提供一定指导。
Objective To investigate the correlation between the abnormal expression of REGγgene and the occurrence,development of papillary thyroid carcinoma( PTC). Methods One hundred and three surgically removing the thyroid specimens in Chengdu Second People’s Hospital from January 2015 to June 2018 were collected. The REGγ mRNA was detected by real-time PCR,the expression of REGγ protein was detected by immunohistochemistry,and they were compared with clinic pathological data. Results In the PTC tissue,the expression level of REGγ mRNA was( 3. 58±1. 60),the positive rate of REGγ protein was 66. 10%,and the above results were all higher than the expression of follicular adenoma of thyroid,struma nodosa and paracancerous normal thyroid tissue( P<0. 05). The expression level of REGγ mRNA and positive rate of REGγ protein in patients with tumor diameter less than 2 cm were significantly higher than those in patients with tumor diameter greater than or equals to 2 cm,the expression level of REGγ mRNA and positive rate of REGγ protein in patients without lymph node metastasis were significantly higher than those in patients with lymph node metastasis( P < 0. 05). There was a positive correlation between the REGγmRNA examination and the expression of REGγ protein in PTC tissues( r = 0. 485,P<0. 05). Conclusion The expression of REGγ gene is associated with the occurrence,development of PTC,which can provide guidance for evaluation of the degree of malignancy,metastasis and prognosis.
作者
涂媛
何林
姚胜平
章培
TU Yuan;HE Lin;YAO Shengping;ZHANG Pei(Department of Pathology, Chengdu Second People’s Hospital, Chengdu Sichuan 610017, China)
出处
《转化医学杂志》
2019年第2期95-97,109,共4页
Translational Medicine Journal
基金
成都市卫计委科研项目(2015121)
