摘要
以黄芩提取物对葡萄座腔菌抑菌圈直径为衡量指标,筛选黄芩的提取工艺条件,对活性物质抑菌稳定性进行研究。结果表明,以80%乙醇作为提取溶剂时,黄芩提取物具有较强抑菌效果,在料液比1∶27.5、超声提取2.5 h、提取温度72.5℃、超声功率850 W条件下,抑菌圈直径达到31.8 mm,相比以丙酮作为提取溶剂,料液比1∶20、在40℃、700 W超声波提取2.0 h条件下的抑菌能力提高了28.69%。紫外光照射10 h以上、偏碱性环境、Ag^+、Fe^(3+)、Cu^(2+)金属离子对提取物活性均产生抑制作用,其中pH值为12时的抑菌圈直径比pH值为5时降低了44.10%;低于25℃储存40 d左右,对提取物活性的影响较小。
Based on antimicrobial circle diameter of Scutellaria baicalensis extract against Botryosphaeria dothidea, the extraction conditions of antibacterial active substances from Scutellaria baicalensis and the antibacterial stability of the active substances were determined. Experimental results show that when 80% ethanol is used as extraction solvent, the extract has strong bacteriostatic effect;the optimum extraction process condition includes ratio of material to liquid 1∶ 27.5, ultrasonic extraction time 2.5 h, extraction temperature 72.5 ℃, and ultrasonic power 850 W, under which the diameter of bacteriostasis circle reached 31.8 mm. The antimicrobial activity of 80% ethanol extract increased by 28.69%, compared to acetone extract in condition with ratio of material to liquid 1∶20, temperature 40 ℃, and 700 W ultrasound for 2.0 h. UV irradiation over 10 h , an alkaline environment, and the presence of metal ions Ag^+, Fe^ 3+, Cu^ 2+ all showed inhibition effects on the activity of Scutellaria baicalensis extract. Compared to a pH 5 environment, the diameter of bacteriostasis circle decreased by 44.10% at pH 12. The activity of extracts was less affected by storage below 25 ℃ for about 40 days.
作者
石浩
王仁才
王琰
庞立
卜范文
何小娥
周倩
SHI Hao;WANG Rencai;WANG Yan;PANG Li;BU Fanwen;HE Xiaoe;ZHOU Qian(College of Horticulture and Landscape,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Agriculture and Forestry,Hunan Applied Technology University,Changde,Hunan 415100,China;College of Orient Science Technology,Hunan Agricultural University,Changsha,Hunan 410128,China;Research Institute of Horticulture in Hunan Province,Changsha 410125,China;College of Plant Protection,Hunan Agricultural University, Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第2期199-204,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科学技术厅重点研发计划项目(2017NK2071
2018NK2013)
关键词
黄芩提取物
提取工艺
葡萄座腔菌
抑菌稳定性
Scutellaria baicalensis extract
extraction process
Botryosphaeria dothidea
antimicrobial stability
