摘要
目的 研究柴胡皂苷D(saikosaponin D,ssd)对急性心肌梗死(acute myocardial infarction,AMI)大鼠血流动力学和心肌氧化应激损伤的保护作用.方法 通过结扎左前降支建立大鼠急性心肌梗死模型,将建模成功的20只雄性大鼠随机分成心梗模型组(AMI)和模型加药组(AMI+SSd),每组10只.20只健康雄性SD大鼠随机分成健康对照组(Ctrl)和单独加药组(SSd),每组10只.单独加药组和模型加药组大鼠腹腔注射50 mg/kg,健康对照组和心梗模型组大鼠腹腔注射等量生理盐水,每天1次,连续2周.多道生理记录仪检测各组大鼠平均动脉压(mean arterial pressure,MAP)、 心率(heart rate,HR)和左心室收缩压(left ventriculoar systolic,LVSP).三苯基氯化四氮唑(2,3,5-Triphenyltetrazolium Chloride,TTC)染色计算心肌梗死面积.苏木伊红(hematoxylin and eosin,HE)染色观察心肌病理损伤.免疫组化检测Ki67的表达.末端标记法(terminal-deoxynucleoitidyl transferase mediated nick end labeling,TUNEL)染色观察心肌细胞凋亡情况.Western印迹检测心肌组织中肌红蛋白(myohemoglobin,Mb)、 肌酸激酶同工酶(creatine kinase-MB,CK-MB)和心肌肌钙蛋白Ⅰ(cardiac troponinⅠ,cTnⅠ)的表达.试剂盒检测线粒体氧化应激指标超氧化物歧化酶(superoxide dismutase,SOD)、 丙二醛(malondialdehyde,MDA)和谷胱甘肽(glutathione,GSH)的含量.结果 与对照组相比,AMI组MAP、HR和LVSP显著降低,心肌梗死面积显著升高;大鼠心肌组织细胞排列紊乱,有大量炎性细胞浸润;Ki67的表达显著下调,凋亡细胞比率显著上升;MB、CK-MB和cTnⅠ的表达显著上调;SOD的活性显著下降,MDA的含量显著上升,GSH的含量显著下降.AMI+SSd组与AMI组相比,MAP、HR和LVSP显著升高,心肌梗死面积显著降低;大鼠心肌组织细胞排列规则,仅有少量炎性细胞浸润;Ki67的表达显著上升,凋亡细胞比率显著下降;MB、CK-MB和cTnⅠ的表达显著下调;SOD的活性显著上升,MDA的含量显著下降,GSH的�
Objective To explore the protective effect of Saikosaponin D on hemodynamics and oxidative stress injury in rats with acute myocardial infarction. Methods The rat model of acute myocardial infarction was established by ligation of the left anterior descending branch, and 20 AMI male rats were randomly divided into two groups (10 rats in each group):The myocardial infarction model group ( AMI) and the model treatment group (AMI + SSd).20 healthy male SD rats were randomly divided into two groups (10 rats in each group):a healthy control group ( Ctrl) and a single drug treatment group (SSd) In the SSd group and the AMI + SSd group, rats were injected with 50 mg/kg Saikosaponin D intraperitoneally. In the healthy control group and the AMI model group, the normal saline was injected into the abdominal cavity, 1 time a day for two weeks. The mean arterial pressure ( MAP), heart rate ( HR) and left ventricular systolic pressure (LVSP) were measured by a multichannel physiological recorder. TTC staining was used to calculate the area of myocardial infarction. HE staining was used to observe the myocardial injury. Immunohistochemistry was used to detect the expression of Ki67. TUNEL staining was employed to observe the apoptosis of cardiac myocytes. Western blotting was utilized to detect the expression of myoglobin (MB), creatine kinase isoenzyme (CK-MB) and cardiac troponin I (cTn I ) in myocardial tissue. Commercial kits were used for the determination of mitochondrial oxidative stress indices :superoxide dismutase (SOD), malondialdehyde ( MDA) and reduced glutathione ( GSH) content. Results Compared with the control group, the MAP, HR and LVSP was decreased significantly and the area of myocardial infarction increased significantly in the AMI group. The myocardial tissue cells in the rats were also in disorder and were infiltrated with a large number of inflammatory cells. The expression of Ki67 was significantly down-regulated;the ratio of apoptotic cells was increased significantly;the expression of MB, CK-MB and c
作者
张洁
艾芬
ZHANG Jie;Al Fen(Department of Emergency, Wuhan Central Hospital, Wuhan, 430000, China)
出处
《医学分子生物学杂志》
CAS
2019年第1期81-86,共6页
Journal of Medical Molecular Biology
基金
湖北省自然科学基金(No.2014CFB324).
关键词
柴胡皂苷D
急性心肌梗死
血流动力学
氧化应激
saikosaponin D
acute myocardial infarction
hemodynamics
oxidative stress
