摘要
目的基于BATMAN-TCM在线分析工具研究钩藤主要活性成分及药理机制。方法从BATMAN-TCM数据库中提取钩藤的化学成分,以Score cutoff≥30和P≤0.05筛选活性成分及作用靶点,分析靶点富集的信号通路与疾病,构建活性成分-靶点-通路-疾病网络,预测钩藤的多维药理机制。结果获得钩藤碱、毛钩藤碱、卡丹宾碱等23个主要活性成分;以及DRD2(多巴胺受体D2基因)、HTR1A(5-羟色胺受体1A)、HTR1B(5-羟色胺受体1B)、SLC18A2等21个核心靶点;活性成分可关联细胞间隙连接、神经活性配体-受体相互作用、钙信号通路等通路11条;可治疗疼痛、心血管系统和神经系统等相关疾病21种。可进一步验证钩藤"多成分—多靶点—多通路"的作用机制。结论本研究从网络药理学角度探讨钩藤主要活性成分、相关通路及多种药理作用机制,为进一步探讨其作用机制奠定了基础。
Objective: To study the main active ingredients and pharmacological mechanism of hookvine from the perspective of network pharmacology. Methods: Chemical constituents of hookvine were extracted from BATMAN-TCM database. Score cutoff ≥ 30 and P ≤ 0.05 were used to screen active constituents and action targets. Signal pathways and diseases of target enrichment were analyzed. Active components-targetpathway- disease network was constructed to predict the multidimensional pharmacological mechanism of hookvine. Results: 23 major active components such as rhynchophylline, irradiata, and kadanbin base were obtained. These components can act on 21 core targets such as DRD2(dopamine receptor D2), HTR1A(serotonin receptor 1A), HTR1B (serotonin receptor 1B), SLC18A2, etc;active ingredients can associat with 11 pathways such as cell gap junction, neuroactive ligand-receptor interaction, and calium signaling pathway;it can treat 21 related diseases such as pain, cardiovascular system and nervous system. The mechanism of “multi-component-multi-target-multi-pathway” of hook-vine can be further verified. Conclusion: In this study, the main active components, related pathways and various pharmacological mechanisms of hookvine were discussed from the perspective of network pharmacology, which laid a foundation for further exploration of its mechanism of action.
作者
段凯旋
罗文浩
罗琼
王博龙
DUAN Kai-xuan;LUO Wen-hao;LUO Qiong;WANG Bo-long(School of Chemical and Biological Engineering,Yichun University,Yichun,Jiangxi 336000,China;Guanghua School of Stomatology,Sun Yat-sen University,Guangzhou,Guangdong 510080,China)
出处
《井冈山大学学报（自然科学版）》
2019年第2期91-96,共6页
Journal of Jinggangshan University (Natural Science)
关键词
钩藤
网络药理学
活性成分
药理作用
作用靶点
hookvine
network pharmacology
active ingredients
pharmacological effects
action target
